Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in Bacillus badius and Bacillus sphaericus Phenylalanine Dehydrogenases
スポンサーリンク
概要
- 論文の詳細を見る
Replacement of glycine by serine at positions 123 and 124 of phenylalanine dehydrogenases from Bacillus badius and Bacillus sphaericus respectively strikingly decreased enzyme activity toward aromatic amino acids and resulted in an elevation of relative activity toward aliphatic amino acids. The mutant from B. badius preferentially dehydrogenated branched-chain amino acids, while that from B. sphaericus acted on amino acids with straight-chain amino acids.
- 社団法人 日本農芸化学会の論文
著者
-
Asano Yasuhisa
Biotechnology Research Center, Toyama Prefectural University
-
Kuwamori Yuko
Biotechnology Research Center, Toyama Prefectural University
-
Tachibana Shinjiro
Biotechnology Research Center, Toyama Prefectural University
関連論文
- Screening, purification, and identification of the enzyme producing N-(L-α-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- High Yield Synthesis of 12-Aminolauric Acid by “Enzymatic Transcrystallization” of ω-Laurolactam Using ω-Laurolactam Hydrolase from Acidovorax sp. T31
- The Screening, Characterization, and Use of ω-Laurolactam Hydrolase: A New Enzymatic Synthesis of 12-Aminolauric Acid
- Purification and Characterization of Maleate Hydratase from Arthrobacter sp. strain MCI2612
- Enzymatic Chemoselective Synthesis of Secondary-Amide Surfactant from N-Methylethanol Amine(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in Bacillus badius and Bacillus sphaericus Phenylalanine Dehydrogenases
- Aldoxime Dehydratase Co-existing with Nitrile Hydratase and Amidase in the Iron-Type Nitrile Hydratase-Producer Rhodococcus sp. N-771 (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- High Yield Synthesis of Nitriles by a New Enzyme, Phenylacetaldoxime Dehydratase, from Bacillus sp. Strain OxB-1(Microbiology & Fermentation Technology)
- Enzymes Involved in Theobromine Production from Caffeine by Pseudomonas putida No.352
- Microbial Production of Theobromine from Caffeine
- Xanthoepocin, a New Antibiotic from Penicillium simplicissimum IFO5762
- Presence of Copalyl Diphosphate Synthase Gene in an Actinomycete Possessing the Mevalonate Pathway
- Improvement in 5'-Position-selective Glucosylation of Pyridoxine by Vertidllium dahliae TPU 4900(Microbiology & Fermentation Technology)
- Screening for New Hydroxynitrilases from Plants
- Structural-Based Engineering for Transferases to Improve the Industrial Production of 5'-Nucleotides
- Improving the Pyrophosphate-inosine Phosphotransferase Activity of Escherichia blattae Acid Phosphatase by Sequential Site-directed Mutagenesis
- Acid Phosphatase/Phosphotransferases from Enteric Bacteria
- A Novel Selective Nucleoside Phosphorylating Enzyme from Morganella morganii
- Nucleotide Sequencing of Phenylalanine Dehydrogenase Gene from Bacillus badius IAM 11059
- Purification and Characterization of A Novel (R)-Hydroxynitrile Lyase from Eriobotrya japonica (Loquat)
- Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in Bacillus badius and Bacillus sphaericus Phenylalanine Dehydrogenases