Screening for New Hydroxynitrilases from Plants

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概要

• 論文の詳細を見る

• We established a simple HPLC method to determine the activity and stereochemistry of the chiral mandelonitrile synthesized from benzaldehyde and cyanide, and applied it to screen for hydroxynitrile lyase (HNL) activity of plant origin. A total of 163 species of plants among 74 families were examined for (R)- and (S)-HNL activities using the method. We discovered that homogenate of leaves of Baliospermum montanum shows (S)-HNL activity, while leaves and seeds from Passiflora edulis, and seeds from Eriobotrya japonica, Chaenomles sinensis, Sorbus aucuparia, Prunus mume, and Prunus persica show (R)-HNL activity. Partially purified (R)-HNLs from Passiflora edulis and Eriobotrya japonica acted not only on benzaldehyde but also on aliphatic ketone. The enantiomeric excess of (R)-methylpropylketone cyanohydrin synthesized from 2-pentanone using homogenate from leaves of Passiflora edulis was 87.0%, and that of (R)-mandelonitrile synthesized by homogenate from seeds of Eriobotrya japonica was 85.0%.

• 社団法人 日本農芸化学会の論文
• 2005-12-23

著者

• Asano Yasuhisa

  Biotechnology Research Center, Toyama Prefectural University

• Tamura Ken'ichirou

  Biotechnology Research Center Toyama Prefectural University

• Asano Y

  National Inst. Genetics Shizuoka

• Asano Yasuhisa

  Biotechnology Res. Center Toyama Prefectural Univ.

• DOI Nobutaka

  Biotechnology Research Center, Toyama Prefectural University

• UEATRONGCHIT Techawaree

  Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University

• H-KITTIKUN Aran

  Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University

• OHMIYA Tohru

  Botanic Gardens of Toyama

• Doi Nobutaka

  Biotechnology Research Center Toyama Prefectural University

• H-kittikun Aran

  Department Of Industrial Biotechnology Faculty Of Agro-industry Prince Of Songkla University

• Asano Yasuhisa

  Biotechnology Res. Center And Dep. Of Biotechnology Toyama Prefectural Univ.

• Ueatrongchit Techawaree

  Department Of Industrial Biotechnology Faculty Of Agro-industry Prince Of Songkla University

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