Isolation of Poly(3-Hydroxybutyrate) (PHB)-degrading Microorganisms and Characterization of PHB-depolymerase from Arthrobacter sp. strain W6
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概要
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Microbial degraders of poly(3-hydroxybutyrate) (PHB) were isolated from soil. Arthrobacter sp. strain W6 used not only PHB as a carbon source, but also PHAs such as poly(3-hydroxybutyrate-co-[5%]3-hydroxyvalerate, poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate), and poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate). PHB-depolymerase was purified to homogeneity from the culture broth of Arthrobacter sp. strain W6 by a procedure involving DEAE- and butyl-Toyopearl column chromato-graphies. The M_r of the enzyme was estimated to be about 47,000 by SDS-polyacrylamide gel electrophoresis. The enzyme was most active at pH 8.5 and 50℃, and was inhibited by phenylmethylsulfonyl fluoride Hg^<2+>, Ag^+, and Pb^<2+>.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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Asano Yasuhisa
Biotechnology Research Center Toyama Prefectural University
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Asano Yasuhisa
Biotechnology Res. Center And Dep. Of Biotechnology Toyama Prefectural Univ.
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WATANABE Sizuko
Biotechnology Research Center, Toyama Prefectural University
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Watanabe Sizuko
Biotechnology Research Center Toyama Prefectural University
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