High Yield Synthesis of Nitriles by a New Enzyme, Phenylacetaldoxime Dehydratase, from Bacillus sp. Strain OxB-1(Microbiology & Fermentation Technology)

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概要

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• 3-Phenylpropionitrile was synthesized from Z-3-phenylpropionaldoxime (0.75 M) in a quantitative yield (98 g/1) by the use of cells of Escherichia coli JM 109/pOxD-9OF, a transformant harboring a gene for a new enzyme, phenylacetaldoxime dehydratase, from Bacillus sp. strain OxB-1. Other arylalkyl- and alkyl-nitriles were also synthesized in high yields from the corresponding aldoximes. Moreover, 3-phenylpropionitrile was successfully synthesized by the recombinant cells in 70 and 100% yields from 0.1 M unpurified E/Z-3-phenylpropionaldoxime, which is spontaneously formed from 3-phenylpropionaIdehyde and hydroxylamine in a butyl acetate/water biphasic system and aqueous phase, respectively.

• 社団法人日本農芸化学会の論文
• 2001-12-23

著者

• Asano Yasuhisa

  Biotechnology Research Center, Toyama Prefectural University

• Xie Sheng-xue

  Biotechnology Research Center Faculty Of Engineering Toyama Prefectural University

• Asano Y

  Department Of Immunology And Host Defenses Ehime University Graduate School Of Medicine

• Asano Y

  Toyama Prefectural Univ. Toyama Jpn

• Kato Y

  Biotechnology Research Center Faculty Of Engineering Toyama Prefectural University

• KATO YASUO

  Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University

• Asano Yasuhisa

  Biotechnology Res. Center And Dep. Of Biotechnology Toyama Prefectural Univ.

• Kato Yasuo

  Biotechnology Research Center And Department Of Biotechnology Toyama Prefectural University

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