Regioselective Glucosylation of Pyridoxine by Microorganisms(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Microorganisms from culture collections and isolates from nature were screened for the ability to catalyze the regioselective glucosylation of pyridoxine (PN) to produce pyridoxine 5'-α-D-glucoside (PN-5'-α-G) or pyridoxine 4'-α-D-glucoside (PN-4'-α-G). Transglucosylation activity specific to 5'-position of PN was found in fungi belonging to genera such as Coriolus and Verticillium, and activity at the 4'-position of PN was found in bacteria belonging to genera such as Bacillus and Serratia. From 100 mM PN, intact cells of Verticillium dahliae TPU 4900 produced 42 mM (13.9 mg/mL) PN-5'-α-G after 70 h of reaction. Intact cells of Bacillus cereus TPU 5504 produced 33mM (10.9 mg/mL) PN-4'-α-G after 19 h of reaction. The seleetivities for 5'- and 4'-positions were 80% and 90%, respectively.
- 社団法人日本農芸化学会の論文
- 2003-03-23
著者
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Wada K
Kawanishi Pharma Research Institute Nippon Boehringer Ingelheim Co. Ltd.
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Wada K
Nagoya Inst. Technol. Nagoya‐shi Jpn
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WADA Koichi
Biotechnology Research Center, Toyama Prefectural University
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Wada K
Biotechnology Research Center Toyama Prefectural University
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Asano Yasuhisa
Biotechnology Res. Center And Dep. Of Biotechnology Toyama Prefectural Univ.
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Wada Koichi
The Department Of Electrical And Computer Engineering Nagoya Institute Of Technology
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Wada Koichi
Biotechnology Research Center Toyama Prefectural University
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