Purification of the Enzymes Responsible for the Lysis of Yeast Cells by Rarobacter faecitabidus(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
We purified yeast-lytic enzymes from Rurobacter faecitabidus, a yeast-lytic coryneform bacterium isolated from a wastewater treatment system. The enzymes consisted of one β-1,3-glucanase and two proteases. Molecular weights of the two proteases and β-1,3-glucanase were 35,000, 33,000, and 82,000 respectively by SDS polyacrylamide gel electrophoresis. The two proteases were serine proteases, which were inhibited by diisopropyl fluorophosphate or phenylmethylsulfonyl fluoride, and most active in alkaline pH. These proteases could decrease the turbidity of yeast suspensions. By this treatment, almost all yeast cells tested lost their viability, although the number of yeast cells did not decrease under microscopic observation. The β-1,3-glucanase was most active at pH5. This enzyme alone neither decreased the turbidity of yeast suspension nor affected the viability of yeast cells, but the β-1,3-glucanase combined with one of the two proteases lysed yeast cells completely.
- 社団法人日本農芸化学会の論文
- 1991-02-23
著者
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SATO S
University of Tsukuba
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SHIMOI Hitoshi
National Research Institute of Brewing
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Shimoi H
Hiroshima Univ. Higashihiroshima Jpn
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Shimoi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shimoi Hitoshi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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SAITO Kazuo
National Research Institute of Brewing
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SATO Shun-ichi
National Research Institute of Brewing
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TADENUMA Makoto
National Research Institute of Brewing
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MURANAKA Yasuhito
National Research Institute of Brewing
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Tadenuma M
Atr Media Information Sci. Lab. Kyoto Jpn
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Tadenuma Makoto
National Reseach Insitute Of Brewing
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Saito K
Yamanashi Univ. Yamanashi Jpn
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Saito Kazuo
National Research Institute For Metals
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