Properties of the Peptides Liberated from Rice Protein in Sokujo-moto
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概要
- 論文の詳細を見る
In the supernatant of sokujo-moto, a high level of acid carboxypeptidase (ACP) activity and a large amount of peptides were observed, however, the amount of free amino acids liberated was small. In order to determine why these peptides were not hydrolyzed to any significant degree by the ACP, the properties of the peptides in sokujo-moto were investigated in this study. Peptides were fractionated from sokujo-moto by ion exchange column chromatography. ACP purified from rice-koji (rice overgrown with Aspergillus oryzae) was allowed to react with the peptides, and it was found that they were not hydrolyzed to any significant degree by the enzyme. Gel filtration chromatography was performed to ascertain the molecular size distribution of the peptides in sokujo-moto, and it was revealed that they were of low molecular sizes; molecular size: mainly in the range of 200-400, and chain length: 2-3. ACP purified from rice-koji was also allowed to react with various synthetic peptides, and it was found that ACP of rice-koji could not rapidly hydrolyze low-molecular-size peptides, such as dipeptides or tripeptides. Acid protease (AP) purified from rice-koji released peptides of molecular sizes mainly in the range of 300-600 or above from rice protein under acidic conditions (pH3.6; the pH of sokujo-moto). When AP and ACP were allowed to act at the same time on rice protein, mainly low-molecular-size peptides (molecular sizes mainly in the range of 200-400) were produced. From these results, it was estimated that AP released peptides with molecular sizes mainly in the range of 300-600 or above from rice protein and ACP degraded the relatively higher molecular size peptides among them to lower molecular size peptides; consequently only low-molecular-size peptides with molecular sizes mainly in the range of 200-400 were released in the supernatant of sokujo-moto.
- 社団法人日本生物工学会の論文
- 1999-09-25
著者
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Furukawa K
General Res. Lab. Of Kiku‐masamune Sake Brewing Co. Ltd. Kobe Jpn
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Yamada T
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Yamada Tasuku
General Res. Lab. Of Kiku-masamune Sake Brewing Co. Ltd.
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Yamada Tasuku
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Takahashi T
Brewing Research Laboratories Sapporo Breweries Ltd.
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Takahashi Toshinari
General Research Laboratories Of Kiku-masamune Sake Brewing Co.ltd.
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Takahashi Toshinari
General Research Laboratory Kiku-masamune Sake Brewing Co. Ltd.
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Furukawa K
Kiku‐masamune Sake Brewing Co. Ltd. Hyogo Jpn
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Furukawa K
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Hara S
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Hara Shodo
Office Of Technical Officers Osaka Regional Taxation Bureau
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Hara Shodo
General Research Laboratories Of Kiku-masamune Sake Brewing Co. Ltd.
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IEMURA YOSHITSUGU
General Research Laboratories of Kiku-Masamune Sake Brewing Co.Ltd.
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FURUKAWA KEIJI
General Research Laboratories of Kiku-Masamune Sake Brewing Co.Ltd.
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Furukawa Keiji
General Research Laboratories Of Kiku-masamune Sake Brewing Co. Ltd.
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Takahashi T
General Research Laboratory Kiku-masamune Sake Brewing Co. Ltd.
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Hara Shodo
General Research Labolatories Of Kiku-masamune Sake Brewing Co. Ltd.
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