A New Rapid Technique for Detection of Microorganisms Using Bioluminescence and Fluorescence Microscope Method
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概要
- 論文の詳細を見る
The MicroStar-RMDS-SPS(RMDS; Rapid Microbe Detection System) enables detection and determination of the number of microorganisms trapped on a membrane filter based on the ATP-bioluminescence method. However, this system could not provide information about the identity of contaminating species based on the measurement results. Therefore, we developed a new technique for observing microcolonies of contaminants using a fluorescence microscope(Micro Scanner). The coordinates in the image data od the RMDS were converted to the coordinates of the sample stage of the microscope, and a microcolony could be easily observed. A single yeast cell and a microcolony consisting of 20-30 lactic acid bacterial cells could be observed on a membrane filter after measurement using the RMDS.
- 社団法人日本生物工学会の論文
- 2000-05-25
著者
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Shinotsuka Ken
Brewing Research Laboratories Sapporo Breweries Ltd.
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Shinotsuka K
Tokyo Inst. Technol. Yokohama Jpn
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WATARI JUNJI
Brewing Research Laboratories, Sapporo Breweries Ltd.
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Takahashi T
Brewing Research Laboratories Sapporo Breweries Ltd.
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Takahashi Toshinari
General Research Laboratories Of Kiku-masamune Sake Brewing Co.ltd.
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Takahashi Toshinari
General Research Laboratory Kiku-masamune Sake Brewing Co. Ltd.
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Watari Junji
Frontier Laboratories Of Value Creation Sapporo Breweries
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Takahashi T
General Research Laboratory Kiku-masamune Sake Brewing Co. Ltd.
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TAKAHASHI TOSHIHIRO
Brewing Research Laboratories, Sapporo Breweries Ltd.
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NAKAKITA YASUKAZU
Brewing Research Laboratories, Sapporo Breweries Ltd.
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Nakakita Yasukazu
Brewing Research Laboratories Sapporo Breweries Ltd.
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