Analysis of an Inactivated Lg-FLO1 Gene Present in Bottom-Fermenting Yeast
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概要
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During the course of investigating a flocculation-related gene of a bottom-fermenting yeast, we identified a new Lg-FLO1 homologue which contains the N-terminal domain of the Lg-FLO1 gene. The results of the partial DNA sequence analysis of the amplified product obtained by inverse-PCR suggested that the homologue contains a sequence present in the YIL169c (chr. IX of Saccharomyces cerevisiae). Southern blot analyses using the VTH1, HXT12, SDL1 and UBP7 genes as probes for chr. IX strongly indicated that an approximately 20-kb region from the YIL169c ORF to the left telomere in chr. IX translocated to the Lg-FLO1 ORF region in chr. VIII of bottom-fermenting yeast. This translocation might convert a flocculent cell to a non-flocculent one.
- 2002-04-25
著者
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Sato Masahide
Brewing Research Laboratories Sapporo Breweries Ltd.
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WATARI JUNJI
Brewing Research Laboratories, Sapporo Breweries Ltd.
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TAKASHIO MASACHIKA
Brewing Research Laboratories, Sapporo Breweries Ltd.
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Watari Junji
Frontier Laboratories Of Value Creation Sapporo Breweries
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MAEBA Hideo
Brewing Research Laboratories, Sapporo Breweries Ltd.
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Maeba Hideo
Brewing Research Laboratories Sapporo Breweries Ltd.
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Sato M
Brewing Research Laboratories Sapporo Breweries Ltd.
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Takashio M
Frontier Laboratories Of Value Creation Sapporo Breweries Ltd.
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Takashio Masachika
Brewing Research Laboratories Sapporo Breweries Ltd.
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