Cloning and Sequencing of the xynA Gene Encoding Xylanase A of Aspergillus kawachii
スポンサーリンク
概要
- 論文の詳細を見る
We have cloned the xynA gene coding for xylanase A, a major commponent of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A.kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA prodcued xylanase. The genomic DNA was arranged as ten exons and nine introns.
- 社団法人日本農芸化学会の論文
- 1992-06-23
著者
-
ITO KIYOSHI
National Research Institute of Brewing
-
ISHIKAWA TAKEAKI
National Research Institute of Brewing
-
IKEMASU Toru
National Research Institute of Brewing
関連論文
- Tolerance Mechanism of the Ethanol-Tolerant Mutant of Sake Yeast
- Cloning of the xynNB Gene Encoding Xylanase B from Aspergillus niger and Its Expression in Aspergillus kawachii
- Effects of Aldehyde Dehydrogenase and Acetyl-CoA Synthetase on Acetate Formation in Sake Mash
- Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding an Acid-Stable α-Amylase from Aspergillus kawachii
- Overproduction of an α-Amylase/Glucoamylase Fusion Protein in Aspergillus oryzae Using a High Expression Vector
- Construction of an α-Amylase/Glucoamylase Fusion Gene and Its Expression in Saccharomyces cerevisiae
- Characteristics of Koji Prepared from the Transformant of Aspergillus oryzae with the Glucoamylase Gene of Aspergillus shirousamii, and Its Utilization for Sake-Brewing
- Cloning of the α-Amylase cDNA of Aspergillus shirousamii and Its Expression in Saccharomyces cerevisiae
- Mode of α-Amylase Production by the Shochu Koji Mold Aspergillus kawachii(Microbiology & Fermentation Technology)
- Cloning and Analysis of the AWA1 Gene of a Nonfoaming Mutant of a Sake Yeast
- Amplified Fragment Length Polymorphism of the AWA1 Gene of Sake Yeasts for Identification of Sake Yeast Strains(BREWING AND FOOD TECHNOLOGY)
- High Expression of Unsaturated Fatty Acid Synthesis Gene OLE1 in Sake Yeasts(Brewing and Food Technology)
- A hapl Mutation in a Laboratory Strain of Saccharomyces cerevisiae Results in Decreased Expression of Ergosterol-Related Genes and Cellular Ergosterol Content Compared to Sake Yeast(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization of α-Ketog1utarate-Resistant Sake Yeast Mutant with High Organic Acid Productivity
- Extracellular Soluble Polysaccharide (ESP) from Aspergillus kawachii Improves the Stability of Extracellular β-Glucosidases (EX-1 and EX-2) and Is Involved in Their Localization
- Production and Some Properties of Salt-Tolerant β-Xylosidases from a Shoyu Koji Mold, Aspergillus oryzae in Solid and Liquid Cultures
- Purification and Characterization of Extracellular and Cell Wall Bound β-Glucosidases from Aspergillus kawachii
- Volatile Compounds Produced by the Fungus Aspergillus oryzae in Rice Koji and Their Changes during Cultivation
- Mycelia Distribution in Rice Koji
- Effect of Processing on the Pore Size Distribution and Digestibility of Rice Grain(Food & Nutrition)
- Retention of Aroma Components in Liquors with Cyclodextrins(Food & Nutrition)
- Purification and Characterization of β-1, 6-Glucanase of Streptomyces rochei Application in the Study of Yeast Cell Wall Proteins(Biochemistry & Molecular Biology)
- Comparison of Acid-Stable α-Amylase Production by Aspergillus kawachii in Solid-State and Submerged Cultures
- Characteristics of Acid-Stable α-Amylase Production by Submerged Culture of Aspergillus kawachii
- Cloning and Sequence Analysis of Endoglucanase Genes from an Industrial Fungus, Aspergillus kawachii(Microbiology & Fermentation Technology)
- Molecular Cloning and Application of a Gene Complementing Pantothenic Acid Auxotrophy of Sake Yeast Kyokai No.7
- Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii
- Cloning and Sequencing of the xynC Gene Encoding Acid Xylanase of Aspergillus kawachii
- Cloning and Sequencing of the xynA Gene Encoding Xylanase A of Aspergillus kawachii
- Isolation and Characterization of Starch-utilizing Mutants of Escherichia coli