Characteristics of Acid-Stable α-Amylase Production by Submerged Culture of Aspergillus kawachii
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概要
- 論文の詳細を見る
A culture medium having a suitable composition for acid-stable α-amylase (asAA) production by submerged culture of Aspergillus kawachii IFO4308 (shochu white-koji mold) is described. Using the proposed submerged medium, approximately 130 mg of asAA protein per liter of the medium were produced at 30℃ for 5 d cultivation. The mold had glycogen as the storage polysaccharide and when the content of storage glycogen (CSG)began to decrease in the presence of an inducer such as dextrin, the production of asA always began. A high level of asAA was produced after production began ans when CSG decreased virtually to zero, however, when CSG started to increase, asAA production tended to be repressed. CSG was affected by both the glucose concentration of the medium and the growth rate of the mycelia. The amount of asAA produced was approximately proportional to the amount of mycelia which had grown after asAA production began.
- 社団法人日本生物工学会の論文
- 1993-08-25
著者
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SUDO SHIGETOSHI
National Research Institute of Brewing
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TAKAYASU-SAKAMOTO YUKO
National Research Institute of Brewing
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ISHIKAWA TAKEAKI
National Research Institute of Brewing
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OBA TOSHITERU
National Research Institute of Brewing
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SATO KAZUO
National Research Institute of Brewing
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Sato K
Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka
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Shiobara Katsumi
Satake Chemical Equipment Mfg. Ltd.
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