Generation of Monoclonal Antibodies Using Simplified Single-Cell Reverse Transcription-Polymerase Chain Reaction and Cell-Free Protein Synthesis(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
The single-step PCR amplification of IgG Light chain (Lc) and Heavy chain (He) (Fd portion) from the cDNAs of a single cell was facilitated using a low concentration of cDNA-specific primers with 5' homotags in the presence of a homotag-specific primer. This method was found to be successful in generating a functional antibody with an antigen-binding activity and useful for the high-throughput generation or screening of monoclonal antibodies.
- 社団法人日本生物工学会の論文
- 2006-03-25
著者
-
Nakano Hideo
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
-
Ali Muhamad
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
-
Ali Muhamad
Lab. Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya Univ. Furo-cho Ch
-
Nakano Hideo
Lab. Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya Univ. Furo-cho Ch
-
Hitomi Kiyotaka
Laboratory of Molecular and Cellular Regulation, Graduate School of Bioagricultural Sciences, Nagoya
-
Ali Muhamad
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
-
Nakano Hideo
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
-
Hitomi Kiyotaka
Laboratory Of Molecular And Cellular Regulation Graduate School Of Bioagricultural Sciences Nagoya U
-
Hitomi Kiyotaka
Laboratory Of Molecular And Cellular Regulation Department Of Applied Molecular Biosciences Graduate
-
Nakano Hideo
Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag
関連論文
- High-throughput screening of DNA binding sites for transcription factor AmyR from Aspergillus nidulans using DNA beads display system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Directed evolution of angiotensin II-inhibiting peptides using a microbead display(METHODS)
- In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis(MEDICAL BIOTECHNOLOGY)
- Stabilization of Affinity-Tagged Recombinant Protein during/after Its Production in a Cell-Free System Using Wheat-Germ Extract
- Directed evolution of angiotensin II-inhibiting peptides using a microbead display
- Synthesis of Phospholipids Containing Polyunsaturated Fatty Acids by Phospholipase A2-mediated Esterification with Food-compatible Reagents
- In Vitro Construction and Screening of a Burkholderia cepacia Lipase Library Using Single-Molecule PCR and Cell-Free Protein Synthesis(METHODS)
- Single-Step Single-Molecule PCR or DNA with a Homo-Priming Sequence Using a Single Primer and Hot-Startable DNA Polymerase
- 1B17-3 A Novel Compact Disk-like Microfluidic Device with Micropillars for Enzyme-linked Immunosorbent Assay
- Improvements in the Cell-Free Production of Functional Antibodies Using Cell Extract from Protease-Deficient Escherichia coli Mutant (Methods)
- EP-P19 Beads display : Genotype-phenotype coupling screening of DNA molecules displayed on microbeads by using emulsion PCR and in vitro protein synthesis(Section VII Enzyme and Protein Engineering)
- Generation of Monoclonal Antibodies Using Simplified Single-Cell Reverse Transcription-Polymerase Chain Reaction and Cell-Free Protein Synthesis(METHODS)
- 3C13-4 Generation and Screening of Monoclonal Antibodies Using Single-Step Single Cell RT-PCR linked in vitro Expression
- Modulator-Mediated Synthesis of Active Lipase of Pseudomonas sp.109 by Escherichia coli Cell-Free Coupled Transcription/Translation System
- Accumulation of Translational Inhibitor durig Multi-Hour Cell-Free Protein Synthesis Reaction Using Rabbit Reticulocyte Lysate
- High Speed Polymerase Chain Reaction in Constant Flow
- In Vitro Selection of DNA Binding Sites for Transcription Factor, PhaR, from Paracoccus denitrificans Using Genetic Library on Microbeads and Flow Cytometry(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 1F14-1 Enzymatic preparation of enantiomerically pure 2,3-diacylglycerol
- Expression Profiles of Polyhydroxyalkanoate Synthesis-Related Genes in Paracoccus denitrificans
- Cloning and in Vitro and in Vivo Expression of Plant Glutathione S-Transferase Zeta Class Genes(GENETICS,MOLECULAR BIOLOGY,AND GENE ENGINEERING)
- Reduction of Protein Degradation by Use of Protease-Deficient Mutants in Cell-Free Protein Synthesis System of Escherichia coli
- Dosage Effect of Minor Arginyl- and Isoleucy1-tRNAs on Protein Synthesis in an Escherichia coli In Vitro Coupled Transcription/Translation System
- Importance of Disulfide Bridge Formation on Folding of Phospholipase D from Streptomyces antibioticus
- Extracellular Production of Phospholipase D of Streptomyces antibioticus Using Recombinat Escherichia coli
- Prolonged Cell-free Protein Synthesis in a Batch System Using Wheat Germ Extract
- Four Types of Calpastain Isoforms with Distinct Amino-Terminal Sequences Are Specified by Alternative First Exons and Differentially Expressed in Mouse Tissues
- An Increased Rate of Cell-free Protein Synthesis by Condensing Wheat-germ Extract with Ultrafiltration Membranes
- Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Emulsion culture : A miniaturized library screening system based on micro-droplets in an emulsified medium(METHODS)
- High-throughput screening method for promoter activity using bead display and a ligase ribozyme(METHODS)
- 3Cp14 Surface loop deletion as a strategy for enzyme thermostabilization
- High-throughput screening method for promoter activity using bead display and a ligase ribozyme