Synchronized Disruption of Excherichia coli Cells by T4 Phage Infection

概要

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For development of an autolytic Escherichia coli protein expression system, T4 bacteriophage (T4)-mediated E. coli disruption was investigated. At least two types of E. coli cell lysis, "lysis from without" (LO) and "lysis from wihtin" (LI), are known to be induced by T4. The efficiency of cell disruption was monitored by the release of β-galactosidase from the cells. In the case of multiplicity of infection (m.o.i.) or 100,the infected cells were lysed without proliferation of the progeny phage (LO). When the cells were infected at a m.o.i. of 5,slow cell lysis (LI) was observed. The β-galactosidase activity detected in the supernatant of the culture subjected to LO was the same as that in the lysate produced by chloroform treatment or sonication of the T4-uninfected culture, but about twice that in the supernatant of the culture subjected to LI. At a m.o.i. of 0.01,delayed onset of cell lysis, called lysis inhibition (LIN), was observed. However, the cells in LIN state were simultaneously lysed upon shifting of the temperature from 37℃ to 0℃, which was accompanied by an increase in extracellular β-galactosidase activity.
社団法人日本生物工学会の論文
1997-06-25