Rhodococcus aetherivorans IAR1, a new bacterial strain synthesizing poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
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Toluene is the volatile organic compound (VOC) that is most abundantly emitted to the environment. Here, we report a new bacterial strain, IAR1, which synthesizes polyhydroxyalkanoate (PHA) from toluene as the sole carbon source. Using physiological tests and phylogenetic analysis, IAR1 was identified as Rhodococcus aetherivorans. Degradation of toluene by resting IAR1 cells followed Michaelis-Menten kinetics. The determined kinetic parameters showed that the degradation ability of IAR1 is slightly lower than that of Acinetobacter sp. Tol 5, which is highly effective for removing toluene from off-gas. PHA synthesized by IAR1 was identified as poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by ^1H NMR and gas chromatography. During fed-batch cultivation of resting IAR1 cells, the principal monomer unit shifted from 3-hydroxybutyrate to 3-hydroxyvalerate. PHBV shows flexibility and toughness comparable to conventional thermoplastics, but its production usually requires supplying expensive precursors as a secondary carbon source. Therefore, employment of R. aetherivorans IAR1 in the production of PHBV from toluene as the sole carbon source is an attractive means for production of the practical copolymer. In addition, the utilization of waste toluene as a raw material for the polymer production will achieve further cost reduction alongside the effective utilization of waste material.
- 社団法人日本生物工学会の論文
著者
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IKEDA Hiroshi
Department of Neurosurgery, Japanese Red Cross Nagoya Daiichi Hospital
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KOBAYASHI Atsushi
Department of Chemistry, Kyushu University
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Hori Katsutoshi
Department of Materials Science and Engineering, Nagoya Institute of Technology
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UNNO HAJIME
Department of Bioengineering, Tokyo Institute of Technology
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Unno Hajime
Dep. Of Biotechnology Tokyo Inst. Of Technol. Yokohama 226-8501 Jpn
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Unno Hajime
Department Of Biotechnology Tokyo Institute Of Technology
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Unno Hajime
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Hori Katsutoshi
Department Of Materials Science And Engineering Nagoya Institute Of Technology:project Research Cent
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Hori Katsutoshi
Department Of Materials Science And Engineering Nagoya Institute Of Technology:project Research Cent
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Kobayashi Atsushi
Department Of Applied Chemistry The University Of Tokyo
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Kobayashi Atsushi
Department Of Biotechnology Tokyo Institute Of Technology
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Kobayashi Atsushi
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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Ikeda Hiroshi
Department Of Gastroenterology & Hepatology Okayama University Graduate School Of Medicine Denti
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Ikeda Hiroshi
Department Of Applied Chemistry Graduate School Of Engineering Osaka Prefecture University
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Ikeda Hiroshi
Department Of Biotechnology Tokyo Institute Of Technology
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Hori Katsutoshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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IKEDA HIROSHI
Deparment of Biology, Faculty of Science, Tokyo Metropolitan University
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