Production of triacylglycerol and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by the toluene-degrading bacterium Rhodococcus aetherivorans IAR1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) produced by various bacteria has been intensively investigated as a promising biodegradable plastic, but required a supply of an expensive precursor as a secondary carbon source for its production. In a previous study, we identified a new bacterial strain, Rhodococcus aetherivorans IAR1, which synthesizes PHBV from toluene without the supply of a precursor. Toluene is the volatile organic compound most abundantly emitted to the environment. In the present paper, we show that R. aetherivorans IAR1 produces triacylglycerols (TAGs) simultaneously with PHBV. Both PHBV and TAGs were synthesized before the nitrogen source is completely exhausted. The cellular content of PHBV reached 10% of cell dry weight (CDW) and its synthesis ceased even during intermittent supply of toluene. However, accumulation of TAGs continued during cultivation and their cellular content reached 24% of CDW at the end of cultivation. Cerulenin inhibited TAG production and increased PHBV cellular content up to 30% of CDW. The mole fraction of 3-hydroxyvalerate (3HV) in PHBV produced from toluene increased from 60% to 80% during its accumulation. Fatty acid compositions of TAGs produced from acetate and toluene were different. At the end of cultivation, the mole fraction of C17:0, one of odd-carbon number fatty acids, was 5% on toluene or 10% on acetate while the mole fraction of 3HV in PHBV from toluene was as high as that in PHBV from acetate, suggesting that a C5 intermediate of toluene degradation might directly become a precursor of 3HV whereas propionyl-CoA is required for the incorporation of C17:0 into TAGs.
- 社団法人日本生物工学会の論文
著者
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Hori Katsutoshi
Department of Materials Science and Engineering, Nagoya Institute of Technology
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UNNO HAJIME
Department of Bioengineering, Tokyo Institute of Technology
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Unno Hajime
Dep. Of Biotechnology Tokyo Inst. Of Technol. Yokohama 226-8501 Jpn
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Unno Hajime
Department Of Environmental Chemical Engineering Kogakuin University
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Unno Hajime
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Hori Katsutoshi
Department Of Materials Science And Engineering Nagoya Institute Of Technology:project Research Cent
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Abe Michiharu
Department of Materials Science and Chemical Engineering, Shizuoka University
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Abe Michiharu
Department Of Materials Science And Chemical Engineering Shizuoka University
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Hori Katsutoshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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