Evaluation of adhesiveness of Acinetobacter sp. Tol 5 to abiotic surfaces(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The toluene-degrading bacterium Acinetobacter sp. Tol 5 shows a highly adhesive and autoagglutinating nature through cell surface nanofibers. In the present study, various aspects of the adhesiveness of Tol 5 cells were evaluated, namely, the association with biofilm formation, the specificity, and the effects of additives. During growth, Tol 5 and Pseudomonas aeruginosa PAO1 cells showed high capacities to form biofilms. However, resting PAO1 cells barely adhered to a polystyrene (PS) surface, while a large number of resting Tol 5 cells rapidly adhered. This implies that Tol 5 cells are intrinsically adhesive and that their initial attachment ability is quite high and distinguishable from their ability to form biofilm. This high adhesiveness of Tol 5 cells was considered to be nonspecific because the cells adhered to various material surfaces from hydrophobic plastic surfaces to hydrophilic glass and metal surfaces. However, Tol 5 cells were found not to be adhesive to Escherichia coli cells. Although Tol 5 cells were capable of interspecies interaction and coagglutination with E. coli cells, at the surface of cell clumps, E. coli cells localized, suggesting that they disturbed autoagglutination of Tol 5 cells. Tol 5 has a hydrophobic cell surface. However, the addition of nonionic surfactant Triton X-100 and bovine serum albumin increased the adhesion of Tol 5 cells to a PS surface, in contrast to previous reports of hydrophobic bacteria. The results highlighted the interesting features of adhesiveness of Tol 5 cells.
- 公益社団法人日本生物工学会の論文
著者
-
Hori Katsutoshi
Department Of Materials Science And Engineering Nagoya Institute Of Technology:project Research Cent
-
Suzuki Atsuo
Department Of Biotechnology Graduate School Of Engineering Nagoya University
-
Shigemori Kazuki
Department Of Chemistry Graduate School Of Science Tohoku University
-
Ishikawa Masahito
Department Of Biotechnology Graduate School Of Engineering Nagoya University
-
Shigemori Kazuki
Department Of Materials Science And Engineering Nagoya Institute Of Technology
-
Hori Katsutoshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
-
Ishikawa Masahit
Department of Biotechnology, Graduate School of Engineering, Nagoya University
関連論文
- Rapid Conversion of Toluene by an Acinetobacter sp. Tol 5 Mutant Showing Monolayer Adsorption to Water-Oil Interface(ENVIRONMENTAL BIOTECHNOLOGY)
- Fate of Coliphage in a Wastewater Treatment Process
- Structural Analysis of a Biofilm Which Enhances Carbon Steel Corrosion in Nutritionally Poor Aquatic Environments
- Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- A Thiourea-Functionalized Benzo-15-crown-5 for Cooperative Binding of Sodium Ions and Anions
- Severe hemophilia A in a Japanese female caused by an F8-intron 22 inversion associated with skewed X chromosome inactivation
- Comparison of Molecular Packing between Two Kinds of Hen Egg-White Lysozyme Orthorhombic Crystals
- Seasonal Changes of Coliphage in Septic Tank Sludge and Quantification of P1 Phage Mediated Gene Transfer
- Rhodococcus aetherivorans IAR1, a new bacterial strain synthesizing poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene(ENVIRONMENTAL BIOTECHNOLOGY)
- Production of triacylglycerol and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by the toluene-degrading bacterium Rhodococcus aetherivorans IAR1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Efficient Ion-Pair Extraction of Potassium Chloride from Aqueous Phase into Nitrobenzene Containing Dicyclohexyl-18-crown-6 as Cation Binder and Diphenylthiourea as Anion Binder
- Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cells affected by growth substrates(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Crystal Structure of Bovine Trypsin and Wheat Germ Trypsin Inhibitor (I-2b) Complex (2 : 1) at 2.3 A Resolution
- Crystallization and Preliminary X-Ray Analysis of a Truncated Family A Alkaline Endoglucanase Isolated from Bacillus sp.
- Crystal Structure of Streptomyces erythraeus Trypsin at 1.9 Å Resolution
- Crystal Structure Analysis of Phospholipase A_2 from Trimeresurus flavoviridis (Habu Snake) Venom at 1.5 A Resolution
- ON THE AIRLINE HUB PROBLEM : THE CONTINUOUS MODEL
- Dynamic Responses of Ferroelectric Liquid Crystals in the Surface-Stabilized Geometry I : Condensed matter
- ARITHMETIC CODING OF VIDEO SIGNALS BASED ON ITERATION OF MOTION COMPENSATION AND MATCHING PURSUIT(INTERNATIONAL Workshop on Advanced Image Technology 2008)
- Trace Elements with Large Activation Cross Section in Concrete Materials in Japan
- Evaluation of adhesiveness of Acinetobacter sp. Tol 5 to abiotic surfaces(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Rapid Conversion of Toluene by an Acinetobacter sp. Tol 5 Mutant Showing Monolayer Adsorption to Water-Oil Interface
- Evaluation of adhesiveness of Acinetobacter sp. Tol 5 to abiotic surfaces
- SOLVING CONSTRAINED TWO-FACILITY LOCATION PROBLEMS(ISOLDE XII)