cDNA Cloning, Expression, and Mutagenesis of Scytalone Dehydratase Needed for Pathogenicity of the Rice Blast Fungus, Pyricularia oryzae
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概要
- 論文の詳細を見る
We purified scytalone dehydratase from the rice blast fungus, Pyricularia oryzae, and cloned its cDNA on the basis of its amino acid sequence. The deduced amino acid sequence was 62% identical to the scytalone dehydratase from Colletotrichum lagenarium. The expression of this gene was induced transcriptionally in the stationary phase when melanin synthesis occurs. We constructed a heterologous expression system for the enzyme in Escherichia coli, did deletion analysis with this system, and found that a Cterminal region is essential for the enzyme function.
- 社団法人日本農芸化学会の論文
- 1998-03-23
著者
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YAMAGUCHI Isamu
The institute of Physical and Chemical Research
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Yamaguchi Isamu
The Institute Of Physical And Chemical Research (riken)
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Motoyama Takayuki
The Institute Of Physical And Chemical Research (riken)
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IMANISHI Kinya
Yuki Research Centre, Nihon Bayer Agrochem Co., Ltd.,
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Imanishi Kinya
Yuki Research Centre Nihon Bayer Agrochem Co. Ltd.
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