Active Center and Mode of Reaction of Blasticidin S Deaminase

スポンサーリンク

概要

• 論文の詳細を見る

• Blasticidin S deaminase (EC 3.5.4.23) was purified by affinity chromatography using a column of Sepharose 4B coupled with pyrimidinoblasticidin S as a ligand. The Michaelis constant Km and the maximum velocity Vmax varied with pH, which suggests that enzyme ionization is important either for substrate binding or for catalytic activity. The inflection point at pH 8.6-8.9 in the plot of pKm versus pH was attributed to an enzyme amino group which corresponded to the carboxyl group of substrates, and an inflection at pH 5.3 in the log Vmax-pH plot was assigned to the imidazole group of histidine, which appeared to be critical for catalytic deaminohydroxylation. The binding of substrates by the enzyme was inferred to be promoted thermodynamically, and activation of the substrate-enzyme complex was presumed to proceed endothermically. From the results obtained, a hypothetical mode of reaction for blasticidin S deaminase is proposed.

• 社団法人 日本農芸化学会の論文

著者

• YAMAGUCHI Isamu

  The institute of Physical and Chemical Research

• MISATO Tomomasa

  The Institute of Physical and Chemical Research

関連論文

• Construction of New ARSI Vectors with Increased Mitotic Stability in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
• RS-22A, B and C:New Macrolide Antibiotics from Streptomyces violaceusniger I.Taxonomy, Fermentation, Isolation and Biological Activities
• Cloning and Expression of a New Structural Gene for Blasticidin S Deaminase, a Nucleoside Aminohydrolase(Biological Chemistry)
• Production of Biodegradable Polyester by a Transgenic Tobacco
• Group I Intron Located in PR Protein Homologue Gene in Youngia japonica
• Liposidolide A, a New Antifungal Macrolide Antibiotic
• Structures of Two Novel Pyriculol-related Compounds and Identification of Naturally Produced Epipyriculol from Pyricularia oryzae(Organic Chemistry)
• cDNA Cloning, Expression, and Mutagenesis of Scytalone Dehydratase Needed for Pathogenicity of the Rice Blast Fungus, Pyricularia oryzae
• Active Center and Mode of Reaction of Blasticidin S Deaminase

スポンサーリンク