Recent Progress in the Generation of Transgenic Medaka (Oryzias latipes)
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概要
- 論文の詳細を見る
Medaka (Oryzias latipes) has been recognized as a potential model vertebrate to which modern molecular genetics can be applied. Recent progress in transgenic techniques emphasizes the importance of the use of medaka in a variety of biological fields, particularly in view of the development of transgenic vectors. The first transgenic medaka was produced by microinjection of DMA into the pronucleus of unfertilized oocytes. Several new methods have since been developed for producing transgenic medaka, and an appropriate method can be selected according to the aims of experiments and application. Transgenic medaka have been used in many fields, including aquaculture, toxicology, developmental biology (phenotypic rescue experiments), and for successful characterization of transcriptional regulatory regions. Here, we describe examples of the application of transgenics in these fields. We also summarize the recent progress in transgenic techniques and transgenic vectors, focusing on the elements and the marker genes that enable identification and/or induction of the expression of exogenous genes in transgenics.
- 社団法人日本動物学会の論文
- 2001-07-01
著者
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Tanaka Minoru
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Kinoshita Masato
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Kinoshita Masato
Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University
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Tanaka Minoru
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Tanaka Minoru
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
関連論文
- CURRENT STATUS OF MEDAKA GENOMICS AND FUTURE PERSPECTIVES(Endocrinology & Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- MEDAKA GENE MAP AND THE VERTEBRATE GENOME EVOLUTION(Endocrinology & Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Synthesis and Tumor-promoting Activities of 12-Epi-phorbol-12,13-dibutyrate
- Expression of the congenital heart disease 5/tryptophan rich basic protein homologue gene during heart development in Medaka fish, Oryzias latipes
- Expression of cardiac myosin light chain 2 during embryonic heart development in medaka fish, Oryzias latipes, and phylogenetic relationship with other myosin light chains
- DMY INDUCE MALE DEVELOPMENT IN XX TRANSGENIC MEDAKA FISH(Endocrinology &Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Solubilization of type I collagen from fish muscle connective tissue by matrix metalloproteinase-9 at chilled temperature
- Cloning and characterization of cDNA for carp matrix metalloproteinase 9
- Possible implication of metalloproteinases in post-mortem tenderization of fish muscle
- Induction of gelatinolytic activities in ayu muscle at the spawning stage
- Gelatinolytic Activities in Ayu Muscle in the Spawning Stage
- cDNA Cloning of Heat-inducible HSP70, a 70.6kDa Heat Shock Protein, in Japanese Flounder Paralichtys olivaceus
- Effect of Steroid Hormone Administration on the Breakdown of Muscle Proteins in Ayu
- Complementary DNA Cloning of HSC71, a 71 kDa Heat Shock Cognate Protein, in Japanese Flounder Paralichtys olivaceus
- Effect of Maturation on the Contents of Free and Bound Forms of Hydroxyproline in Ayu Muscle
- Comparison of Ultrasonic Vocalizations Emitted by Rodent Pups
- Diploidized eggs reprogram adult somatic cell nuclei to pluripotency in nuclear transfer in medaka fish (Oryzias latipes)
- ADULT INDIVIDUALS OBTAINED FROM EMBRYOS RECONSTRUCTED BY NUCLEAR TRANSFER OF THE ADULT CAUDAL FIN CELLS IN MEDAKA(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Structure and Transcription of the Gene Coding for Polypeptide Chain Elongation Factor 1α of Medaka Oryzias latipes
- Sequence and Expression of a cDNA Encoding Japanese Flounder Glucocorticoid Receptor
- cDNA Cloning of Polypeptide Chain Elongation Factor 1α from Medaka Oryzias latipes
- Estrogen inhibits development of yolk veins and causes blood clotting in transgenic medaka fish overexpressing estrogen receptor
- Effects of i. c. v. Administration of Leptin on Copulatory and Ingestive Behavior in STZ-induced Diabetic Male Rats
- A transgene and its expression profile are stably transmitted to offspring in transgenic medaka generated by the particle gun method
- Transgenic Medaka Overexpressing a Melanin-Concentrating Hormone Exhibit Lightened Body Color but No Remarkable Abnormality
- Purification and Characterization of Prophenoloxidase from Kuruma Prawn Penaeus japonicus
- Ploidy mosaicism in well-developed nuclear transplants produced by transfer of adult somatic cell nuclei to nonenucleated eggs of medaka (Oryzias latipes)
- FERTILE AND DIPLOID MEDAKA GENERATED FROM BLASTULA AND 4-SOMITE-STAGE EMBRYONIC CELLS BY NUCLEAR TRANSPLANTATION TO NON-ENUCLEATED OOCYTES(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan
- CHROMOSOMAL STATUS OF EMBRYOS GENERATED BY TRANSPLANTATION OF CULTURED CELL NUCLEI FROM THE CAUDAL FIN TO NONENUCLEATED OOCYTES OF MEDAKA(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Development and gene expression of nuclear transplants generated by transplantation of cultured cell nuclei into non-enucleated eggs in the medaka Oryzias latipes^+
- Acceptability comparison of kamaboko gels derived from silver carp surimi and from walleye pollack surimi between the Chinese and Japanese
- Activity of the medaka translation elongation factor 1α-A promoter examined using the GFP gene as a reporter
- Transplantation of blastula nuclei to non-enucleated eggs in the medaka, Oryzias latipes
- Responses of Embryonic Germ Cells of the Radiation-sensitive Medaka Mutant to γ-irradiation
- Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus
- PRODUCTION OF MATERNAL-ZYGOTIC MUTANT MEDAKA BY PGC TRANSPLANTATION INTO INTERSPECIFIC HYBRID(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- TRANSGENIC MEDAKA (ORYZIAS LATIPES) WITH BRILLIANT FLUORESCENCE IN SKELETAL MUSCLE(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Transgenic medaka with brilliant fluorescence in skeletal muscle under normal light
- Recent Progress in the Generation of Transgenic Medaka (Oryzias latipes)
- Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases