Responses of Embryonic Germ Cells of the Radiation-sensitive Medaka Mutant to γ-irradiation
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概要
- 論文の詳細を見る
Embryonic germ cell/γ-irradiation/Olvas-GFP/ric//Medaka. The radiation-sensitive mutant "ric1" has a defect in the repair mechanism of DNA double strand breaks induced by γ-rays in early embryogenesis. In this study, the new radiation-sensitive Medaka (Oryzias latipes) strain, riclolvas-GFP was established to monitor the development of germ cells in vivo. The development of germ cells was normal in riclolvas-GFP, but embryonic germ cells at Stage 7 (32-cell stage) and Stage 33 (extensive proliferating stage of PGCs) showed higher radiosensitivity. There was no sex difference in germ cell radiosensitivity at Stage 7, but female embryos showed higher radiosensitivity than male at Stage 33. In embryos obtained by crossing ric1 female with olvas-GFP male, germ cells showed similar radiosensitivity to riclolvas-GFP and increased sensitivity compared to embryos obtained from crossing wild-type female with olvas-GFP male at Stage 7. These results suggest that germ cells have the ric1 dependent DNA repair system during embryogenesis and the maternal ric1 gene factor may play a critical role in radiosensitivity at an early developmental stage.
- 2007-03-20
著者
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Mitani Hiroshi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Aizawa Kouichi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo:sa
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Aizawa Kouichi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Aizawa Kouichi
Department Of Electrical Engineering Kyoto University
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Mitani Hiroshi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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Yamashita Toshikazu
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Yamashita Toshikazu
Departmen Of Physiology Nihon University School Of Medicine
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Oda Shoji
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Kinoshita Masato
Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University
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YORI Kanako
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
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KAMINAGA Chiharu
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
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Mitani Hiroshi
Department Of Biological Sciences School Of Science University Of Tokyo.
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Yori Kanako
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Kaminaga Chiharu
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Mitani Hiroshi
Department Of Biological Sciences Graduate School Of Frontier Sciences University Of Tokyo
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Kinoshita Masato
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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