The Effect of Extracellular Ca^<2+> on Responses to Purinoceptor Agonists in Cultured Swine Tracheal Smooth Muscle Cells

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概要

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• The effects of extracellular Ca^<2+> ions on purinergic responses were examined in swine tracheal smooth muscle cells (TSMCs) in primary culture. ATP (1μM to 1mM) and α, β-methylene adenosine 5'-triphosphate (α, β-Me ATP) (100μM and 1mM) concentration-dependently increased [Ca^<2+>]_1 in the presence and the absence of extracellular Ca^<2+>. Responses to ATP (10μM to 1mM) in the presence of extracellular Ca^<2+> were significantly larger than those in its absence (n=8), whereas those to α, β-Me ATP were not significantly different between the presence (n=7) and the absence (n=8) of extracellular Ca^<2+%gt;. Responses to ATP (1mM) at extracellular Ca^<2+> concentration ([Ca^<2+>]_o) of 10 and 5mM were significantly larger than that at extracellular EGTA concentration ([EGTA]_o) of 1mM (p<0.01, n=5), whereas the responses to α, β-Me ATP (1mM) at 10 and 5mM [Ca^<2+>]_o were significantly smaller than that at [EGTA]_o of 1mM (p<0.05, n=5). Increasing [Ca^<2+>]_o to 5mM after the application of either 1mM ATP (n=4) or 1mM α, β-Me ATP (n=4) in the absence of extracellular Ca^<2+> (1mM [EGTA]_0) further increased [Ca^<2+>]_1, though the increases in [Ca^<2+>]_1 by agonists in 1mM [EGTA]_o had been already maximal. Incubating cells for 300s in 5mM [Ca^<2+>]_o before the application of ATP (1mM) significantly increased the response to the drug than that obtained by incubating cells for 6sec in 5mM [Ca^<2+>]_o before the drug application (p<0.01, n=4). However, α, β-Me ATP (1mM) induced similar responses by incubating cells for 30 or 300 s in 5mM [Ca^<2+>]_o to that by incubating them for 6s. These results suggest that the effect of α, β-Me ATP in swine TSMCs in primary culture is mainly through Ca^<2+> release and that its effect on Ca^<2+> entry is smaller than other nucleotides.

• 日本平滑筋学会の論文

著者

• Sawai Hiroko

  Departmen Of Physiology Nihon University School Of Medicine

• Kokubun S

  Departmen Of Physiology Nihon University School Of Medicine

• Kokubun Shinichiro

  Department Of Physiology The Jikei Univ.school Of Medicine

• WANG Ruixia

  Department of Chemistry, Chizhou College

• Kokubun Shinichiro

  Departmen Of Physiology Nihon University School Of Medicine

• Yamashita Toshikazu

  Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo

• Yamashita Toshikazu

  Departmen Of Physiology Nihon University School Of Medicine

• Wang Ruixia

  Departmen Of Physiology Nihon University School Of Medicine

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