Induction and characterization of mutations at the b locus of the medaka, Oryzias latipes(Genetics)
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概要
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The b locus is one of the most familiar pigmentation loci in the medaka, but its biochemical function is still unknown. Here we report induction of new mutations at the b locus by radiation and ENU. We also characterized all these mutations and previously isolated spontaneous ones on the phenotypic basis. Unexpectively, all the 18 induced mutations reduced melanin contents in both eyes and skin correlatively, although degree of reduction was varied from mutations to mutations. Moreover, presumed null mutants (b^<s8>, b^<g8>, b^<c2>, b^<d3>, b^<d6>, b^<g13>, b^<g19>, b^<g24>) had slightly melanized (dark red) eyes. These results suggest that the b-locus product plays an important but not a critical role in melanogenesis. The spontaneous mutants were divided into two types: one (b^<dl2>, b^<dl3>, and b^p) had similarities with the induced mutants in that they had slightly colored eyes and skin, the other (b^v, B', b^d, b^<dl1>, and b) exhibited normally black eyes but lightly colored skin. The present study supports our recent results (Fukamachi et al., 2001) that mutational changes were found in the coding region of the b gene in some of the mutants which reduced both eyes and skin melanogenesis, while the mutational change for the b allele could not be found there. We speculate that the b^v, B', b^d, b^<dl1>, and b alleles might arise by the mutations in the regulatory region for skin melanogenesis.
- 社団法人日本動物学会の論文
- 2002-04-25
著者
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Shima Akihiro
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
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Fukamachi Shoji
epartment of Integrated Biosciences, University of Tokyo
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Shimada Atsuko
Department of Biological Sciences, University of Tokyo
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Fukamachi Shoji
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
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Wakamatsu Yuko
Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University
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Ozato Kenjiro
Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University
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Shima Akihiro
Department Of Molecular Biology Keio University School Of Medicine
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Shimada A
Department Of Molecular Biology Keio University School Of Medicine
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Shimizu Atsushi
さいたま市立病院 皮膚科
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Shima A
Department Of Molecular Biology Keio University School Of Medicine
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Ozato Kenjiro
Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University
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Wakamatsu Yuko
Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University
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Shima Akihiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo.
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Shimada Atsuko
Dept. Biol. Sci. Univ. Tokyo
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Shima Akihiro
Lab. Radiat. Biol. Dept. Biol. Sci. Grad. Sch. Sci. Univ. Tokyo.
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Shimada Atsuko
Department Of Biological Sciences University Of Tokyo
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Fukamachi Shoji
Epartment Of Integrated Biosciences University Of Tokyo
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Fukamachi Shoji
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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Shima Akihiro
Department Of Integrated Biosciences University Of Tokyo
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Shimada Atsuko
Department Of Biological Science Graduate School Of Science University Of Tokyo
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Shima Akihiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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