<SESSION>DOSE-RATE DEPENDENCY OF RADIATION-INDUCED GERM CELL MUTATION : DEVELOPMENT OF A NEW MUTATION ASSAY SYSTEM USING THE FISH, MEDAKA ORYZIAS LATIPES
スポンサーリンク
概要
- 論文の詳細を見る
A new specific-locus mutation assay system using the fish Oryzias latipes has been developed. An advantage of our system is that we can detect deaths around hatching period of many mutant embryos whose mutant phenotypes could be recognized, as "total mutations", early after fertilization. This system has revealed that 80 to 90% of the embryos with total mutations induced in spermatogonia by gamma-rays died of some developmental defects before hatching. We are studying dose-rate dependency of total mutations as well as viable mutations induced in spermatogonia. Although our data are still incomplete, it is indicated that no dose-rate effect was found in total mutations, whereas dose-rate effect exists with regard to viable mutations under the present experimental conditions.
- 核融合科学研究所の論文
著者
-
Shimada Atsuko
Laboratory of Radiation Biology, Department of Biological Sciences, School of Science, University of
-
Shima Akihiro
Department Of Molecular Biology Keio University School Of Medicine
-
Shimada Atsuko
Dept. Biol. Sci. Univ. Tokyo
-
Shima Akihiro
Lab. Radiat. Biol. Dept. Biol. Sci. Grad. Sch. Sci. Univ. Tokyo.
-
Shimada Atsuko
Department Of Biological Sciences University Of Tokyo
-
Shima Akihiro
Department Of Integrated Biosciences University Of Tokyo
-
Shima Akihiro
Laboratory Of Radiation Biology Department Of Biological Sciences The University Of Tokyo
関連論文
- CHARACTERIZATION OF THE MEDAKA Y-CHROMOSOME SPECIFIC REGION(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- The DNA Sequence of Medaka Chromosome LG22(Comparative Genomics of Animals,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine.
- POLYMORPHISM OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I REGION OF MEDAKA, ORYZIAS LA TIPES(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- CURRENT STATUS OF MEDAKA GENOMICS AND FUTURE PERSPECTIVES(Endocrinology & Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- 70 Analysis of Responses of a Radiation Sensitive Medaka Mutant to Gamma-irradiation at Early Developmental Stage(Radiation sensitivities in cells & tissues, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
- MEDAKA GENE MAP AND THE VERTEBRATE GENOME EVOLUTION(Endocrinology & Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Positional cloning of the responsible gene for medaka orangered variants(Symposium on Comparative and Functional Genomics of Medaka)
- Structural and functional characterization of gonadotropinreleasing hormones and their receptors in the medaka Oryzias latipes(Symposium on Comparative and Functional Genomics of Medaka)
- Gamma-ray Accelerates the Spermatogenesis of the Medaka Fish, Oryzias latipes
- A genome-wide screen for mutations affecting embryonic radiation sensitivity in Medaka, Oryzias latipes
- Induction and characterization of mutations at the b locus of the medaka, Oryzias latipes(Genetics)
- Alternative Timing in Responses to γ-irradiation of Medaka Early Embryos
- Critical Stage in Spermatogenesis for γ-ray Induced Cell Death
- Responses of Medaka Early Embryoto γ-irradiation
- γ-Ray Induced Spermatogonial Death of Medaka, Oryzias latipes.
- Chromosome aberrations in F_1 embryos derived from male medaka germ cells exposed to low dose γ-rays
- Detection of genomic alterations in embryos derived from γ-irradiated sperm・spermatids by IRS based PCR
- Sex-Linked Inheritance of the lf Locus in the Medaka Fish(Oryzias latipes)
- Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis
- Role of pyrimidine dimers in UV-induced apoptosis
- Induction and Photorepair of Sunlamp UV-Induced Pyrimidine Dimers in the Early Embryos of the Medaka, Otyzias latipes.
- Expression of photolyase gene in embryos of the Modaka, Otyias latipes.
- Chromosome aberrations in F1 embryos derived from male medaka germ cells exposed to γ-rays
- Detection of Deletion Mutants of CPD-Photolyase Gene Using Medaka by a PCR System
- Visible InGaP / GaAsP Dual Wavelength Light Emitting Diodes
- Genomic alterations in Medaka embryos derived from γ-irradiated sperm spermatids
- Genetic Relationships of the Genus Tridentiger (Pisces, Gobiidae) Based on Allozyme Polymorphism
- (6-4) photolyase in cultured go1dfish cells
- Medaka as a model organism for research in experimental biology
- Radiation-induced Apoptosis Responsible for Limb Teratogenesis in Embryonic Mice
- Radiation Induced Apoptosis in the Mouse Limb Bud Cells in vivo and in vitro
- Induction of photolyase and cellular attachment to substratum byphotosensitizer in combination with light.
- Relationship between survival and (6-4) photoprducts in cultured fish cells.
- The genome size evolution of medaka (Oryzias latipes) and fugu (Takifugu rubripes)
- CYTOFLUOROMETRIC DETERMINATION OF EXCISION REPAIR OF UV-INDUCED PYRIMIDINE DIMERS IN EHRLICH ASCITIC CANCER CELLS
- Effects of β-Radiation from Organically Bound Tritium (ORT) on Cultured Embryonic Mid Brain Cells of Mice
- Germ-cell Mutagenesis in the Medaka Oryzias latipes (X)- DNA Alterations Detected in Total Mutants by AP-PCR/RAPD Method
- Distribution and phylogeny of rice fish and their relatives belonging to the suborder Adrianichthyoidei in Sulawesi, Indonesia
- Gene-Centromere Mapping for 5 Visible Mutant Loci in Multiple Recessive Tester Stock of the Medaka (Oryzias latipes) : COMMUNICATION : Genetics and Immunology
- Effects of UVC-Irradiation on Cultured Mouse Limb Bud Cells
- Repair of (6-4)photoproduas in cultured fish cells.
- Interactions between photoropair and excision repair in cultured fish cells.
- COMBINATION OF FEULGEN NUCLEAR REACTION WITH IMMUNOFLUORESCENT STAINING FOR PHOTOPRODUCTS OF DNA AFTER UV-IRRADIATION
- DOSE-RATE DEPENDENCY OF RADIATION-INDUCED GERM CELL MUTATION : DEVELOPMENT OF A NEW MUTATION ASSAY SYSTEM USING THE FISH, MEDAKA ORYZIAS LATIPES
- Establishment of a Cell Line (PF Line) from a Gynogenetic Teleost, Poecilia formosa (Girard) and Characterization of Its Repair Ability of UV-Induced DNA Damage(Cell Biology)
- Cloning and characterization of cDNAs for 70-kDa heat-shock proteins(Hsp70) from two fish species of the genus Oryzias.