Establishment of a Cell Line (PF Line) from a Gynogenetic Teleost, Poecilia formosa (Girard) and Characterization of Its Repair Ability of UV-Induced DNA Damage(Cell Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• Fibroblast-like cells obtained from explant culture of an one-day-old fry of a naturally occurring gynogenetic teleost, the Amazon molly (Poeciliaformosa), were serially subcultivated with a doubling time of approximately 1.5 days at 27℃ for 540 days when the cells attained 252 Population Doubling Number (PDN) and the serial passage was stopped. No symptom of crisis was encountered during the passage. At several PDNs, changes in various biological parameters were examined. The growth characteristics of the cells strongly indicated that this PF line, like some other cell lines of fish, appears to have unlimited proliferating potentials without any apparent sign of being malignantly transformed, and hence to have been immortalized without transformation. During subcultivation, the cloning efficiencies considerably fluctuated between approx. 1 to 20 %. Although the number of chromosomes was the same (46) at 12, 75, and 175 PDNs, all of the 46 chromosomes were acrocentric at 12 PDN while 45 acrocentric and 1 submetacentric chromosomes were found at 75 and 175 PDNs. The saturation cell density ranged from 6.8 to 12 × 10^4 cells/cm^2 during passage and the contact inhibition of growth generally worked well. The cells were highly proficient in photoreactivation of UV-induced cell killing as well as DNA damage in terms of UV-specific endonuclease sensitive sites. The potential usefulness of this cell line for study of relationships between DNA damage and carcinogenesis was discussed.

• 社団法人日本動物学会の論文
• 1985-08-15

著者

• Shima Akihiro

  Laboratory Of Radiation Biology Zoological Institute Faculty Of Science University Of Tokyo

• Shima Akihiro

  Laboratory Of Radiation Biology Department Of Biological Sciences The University Of Tokyo

• Setlow Richard

  Biology Department Brookhaven National Laboratory

• Setlow R.B.

  Biology Department, Brookhaven National Laboratory

関連論文

• Sex-Linked Inheritance of the lf Locus in the Medaka Fish(Oryzias latipes)
• Role of pyrimidine dimers in UV-induced apoptosis
• Induction and Photorepair of Sunlamp UV-Induced Pyrimidine Dimers in the Early Embryos of the Medaka, Otyzias latipes.
• Expression of photolyase gene in embryos of the Modaka, Otyias latipes.
• Medaka as a model organism for research in experimental biology
• CYTOFLUOROMETRIC DETERMINATION OF EXCISION REPAIR OF UV-INDUCED PYRIMIDINE DIMERS IN EHRLICH ASCITIC CANCER CELLS
• X-Ray Sensitivity of Fibroblast Cell Strains Derived from Atomic Bomb Survivors with and without Breast Cancer : II. BIOLOGICAL EFFECTS
• COMBINATION OF FEULGEN NUCLEAR REACTION WITH IMMUNOFLUORESCENT STAINING FOR PHOTOPRODUCTS OF DNA AFTER UV-IRRADIATION
• DOSE-RATE DEPENDENCY OF RADIATION-INDUCED GERM CELL MUTATION : DEVELOPMENT OF A NEW MUTATION ASSAY SYSTEM USING THE FISH, MEDAKA ORYZIAS LATIPES
• Establishment of a Cell Line (PF Line) from a Gynogenetic Teleost, Poecilia formosa (Girard) and Characterization of Its Repair Ability of UV-Induced DNA Damage(Cell Biology)
• Cloning and characterization of cDNAs for 70-kDa heat-shock proteins(Hsp70) from two fish species of the genus Oryzias.

もっと見る 閉じる

スポンサーリンク