Inhibitory Specificity against Various Trypsins and Stability of Ovomucoid from Japanese Quail Egg White.

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概要

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• The inhibitory specificity and stability of ovomucoid from Japanese quail egg white (OMJPQ) were examined to understand its nutritional significance. OMJPQ showed strong inhibitory activities toward trypsins from various origins including human, and the trypsin inhibitions occurred at molar ratios of enzyme to inhibitor between 1/1 and 2/1. On the other hand, an equimolar mixture of the second and third domains of OMJPQ inhibited bovine trypsin more strongly than the corresponding native OMJPQ did. This distinction was partly explained by the presence of steric hindrance on the formation of a 2:1 trypsin-OMJPQ complex. OMJPQ retained about 100% of its original activity over a pH range from 1 to 12 after a 24-h incubation at 37°C. The inhibitor was most thermostable between pH 2 and 5, where more than 70% of its original activity was maintained after a 1-h incubation at 100°C and about 25% of the activity even after a 30-min incubation at 121°C. OMJPQ was also considerably resistant to pepsin attack. Pepsin digestion of the protein resulted in only about 40% loss of the original trypsin-inhibitory activity even after a 24-h digestion. Furthermore, the addition of bovine serum albumin to the digestion mixture brought about rapid elevation in the trypsin-inhibitory activity during an initial 30-min digestion. SDS-PAGE and immunoblot suggested that this was due to the liberation of active inhibitory domains from the native molecule by inter-domain proteolysis.

• 財団法人 学会誌刊行センターの論文

著者

• Asao Toshio

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Tashiro Misao

  Department Of Food Science And Nutrition Faculty Of Living Science Kyoto Prefectural University

• Takahashi Kyoko

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• KITAO Satomi

  Department of Food Science and Nutrition, Kyoto Prefectural University

• KANAMORI Masao

  Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• KANAMORI Masao

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto Prefectural University

• ASAO Toshio

  Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

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