Purification and Characterization of a Major Trypsin Inhibitor, FMTI-II, from Foxtail Millet Grain

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概要

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• A major trypsin inhibitor was purified from the extract of the whole grain of foxtail millet, Setaria italica, to an electrophoretically homogeneous protein by conventional methods. This inhibitor (FMTI-II) has a molecular weight of 7500 and contains high levels of basic amino acids, acidic amino acids, prolinc, and half-cystine. FMTI-II inhibited bovine and hog trypsins in a 1:1 (M/M) stoichiometry: the Ki values were 3.0 × 10-11 M and 2.2 × 10-10 M, respectively. Bovine α-chymotrypsin, subtilisin BPN, hog pepsin, and papain were not inhibited. The inhibitor was stable in a wide range of pH and was heatresistant at acidic pH. The chemical modification suggested that FMTI-II had a Lys-X bond as a trypsin reactive-site. FMTI-II resembles rice bran and wheat germ trypsin inhibitors, showing that it is a Bowman-Birk type inhibitor.

• 社団法人 日本農芸化学会の論文

著者

• Tashiro Misao

  Department Of Food Science And Nutrition Faculty Of Living Science Kyoto Prefectural University

• Katayama Yoshiho

  Department Of Food And Nutrition Faculty Of The Science Of Living Osaka Clty University

• MAKI Zensuke

  Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University

• KIHIRA Yuko

  Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University

• KATAYAMA Yoshiho

  Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University

関連論文

• Purification, Characterization, and Amino Acid Sequence of Foxtail Millet Trypsin Inhibitor III(Biological Chemistry)
• Purification and Characterization of a Subtilisin Inhibitor from Seeds of Foxtail Millet, Setaria italica(Biological Chemistry)
• Identification of Reactive Site of a Proteinaceous Metalloproteinase Inhibitor from Streptomyces nigrescens TK-23
• Trypsin Hydrolysis of the Tyr(42)-Ser(43) Bond, the Chymotrypsin Reactive-site Peptide Bond, of Faba Bean Bowman-Birk Type Inhibitor
• Purification and Characterization of a Bowman-Birk Type Proteinase Inhibitor from Faba Beans(Vicia faba L.)(Biological Chemistry)
• A MODIFIED TECHNIQUE OF HISTOCHEMICAL DEMONSTRATION FOR N-ACETYL-β-HEXOSAMINIDASE
• Iron Trends in Rat Serum Related to Dietary Fish Oils under Protein Malnutrition
• EFFECTS OF EXERCISE TRAINING ON BODY COMPOSITION OF RATS FED VARIOUS PROTEIN-LEVEL DIETS
• Changes in Activity, Antigenicity, and Molecular Size of Rice Bran Trypsin Inhibitor by In Vitro Digestion
• Isolation of Protein Inhibitors of Papain, Trypsin, and α-Amylase in the Grain of Foxtail Millet
• Inhibitory Specificity against Various Trypsins and Stability of Ovomucoid from Japanese Quail Egg White.
• Rapid Purification of Streptomyces griseus Trypsin by Immobilized Rice Bran Trypsin Inhibitor
• Purification and Characterization of a Major Trypsin Inhibitor, FMTI-II, from Foxtail Millet Grain

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