Availability of Dihydrofolate Reductase Affinity Handle in Expressing Human Prolactin as a Soluble Fusion Protein
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概要
- 論文の詳細を見る
Human prolactin (PRL) cDNA was successfully expressed in Escherichia coli cells with the aid of a dihydrofolate reductase (DHFR) affinity handle. The formed DHFR-PRL fusion protein was accumulated in E. coli cells as a soluble protein with DHFR activity at 30℃. The fusion protein was highly purified with monitored the DHFR activity by methotrexate-bound affinity chromatography, suggesting the usefulness of the handle even in expressing a large polypeptide as a fusion protein.
- 社団法人日本農芸化学会の論文
- 1993-11-23
著者
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Morikawa Minoru
Institute For Production Research And Development The Nikka Whiskey Distilling Co. Ltd.
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Iwakura Masahiro
National Institute of Advanced Industrial Science and Technology
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Iwakura Masahiro
Joint Research Center For Atom Technology(jrcat)national Institute Of Bioscience And Human-technolog
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Iwakura Masahiro
National Institute Of Bioscience And Human-technology
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Iwakura M
Joint Research Center For Atom Technology(jrcat)national Institute Of Bioscience And Human-technolog
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Iwakura Masahiro
National Institute For Advanced Interdisciplinary Research
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