Novel Screening Method for Potential Skin-Whitening Compounds by a Luciferase Reporter Assay
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概要
- 論文の詳細を見る
Measurement of the melanin content by using B16 melanoma cells is generally applied to find novel skin-whitening agents. However, this measurement method using B16 melanoma cells has such disadvantages, as the time taken, its sensitivity, and troublesomeness. We therefore attempted in the present study to establish a reporter assay system by measuring the tyrosinase promoter activity to use for convenient, high-throughput screening of new melanogenesis inhibitors. We first confirmed the validity of this reporter assay system by using such known skin-whitening agents, as arbutin, sulforaphane, and theaflavin 3,3′-digallate. We then compared the effect of 56 compounds on the tyrosinase promoter activity to test this reporter assay system. Carnosol, and rottlerin strongly inhibited the tyrosinase promoter activity. Moreover, carnosol and rottlerin decreased melanin synthesis and tyrosinase expression in a dose-dependent manner when using B16 melanoma cells. These results indicate this new luciferase reported assay system to be an effective and convenient method for screening potential skin-whitening compounds.
- 社団法人 日本農芸化学会の論文
著者
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Sakakibara Yoichi
Interdisciplinary Graduate School Of Agriculture And Engineering Univ. Of Miyazaki
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LIU Ming-Cheh
Department of Pharmacology, College of Pharmacy, The University of Toledo
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榊原 陽一
宮崎大・農・応生科
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YAMASAKI Masao
Department of Cellular and Integrative Physiology, Fukushima Medical University School of Medicine
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NISHIYAMA Kazuo
Department of Pharmacology, Kitasato University School of Medicine
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Shirasugi Ichiro
Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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MATSUI Takashi
Kurakon Co., Ltd.
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SUIKO Masahito
Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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Sakakibara Yoichi
Department Of Forest Science And Resources College Of Bioresource Sciences Nihon University
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