Reactivities and Specific Inhibitory Effects of al, 3, 4-Thiadiazolo[3, 2-a]pyrimidine on SH Enzymes
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概要
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We studied the reactivity of 2-ethylsulfonyl-7-methyl-5H-1, 3, 4-thiadiazolo[3, 2-a]pyrimidin-5-one (TPSO2-2) with some amino acids, SH, OH, and histidine enzymes. TPSO2-2 reacted with lcysteine in high yield (80-90%), but with glycine and L-serine in low yield (below 10%). To clarify the relative reactivity of TPSO2-2, its reaction with L-cysteine in the presence of ethanol and diethylamine was examined. TPSO2-2 reacted only with the SH group of L-cysteine, not with ethanol nor diethylamine. To elucidate the reactivity of TPSO2-2 toward enzymes, the inhibitory effects of TPSO2-2 on some enzymes containing cysteine, serine, or histidine in the active center were investigated. TPSO2-2 showed high inhibitory effects on SH enzymes, such as yeast alcohol dehydrogenase, glutamate dehydrogenase, and hexokinase, but no effect on trypsin, which has serine, or catalase, with histidine in the active center. TPSO2-2 appeared to be a specific inhibitor of SH enzymes.
- 社団法人 日本農芸化学会の論文
著者
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Nakatsu Seiichiro
Department Of Agricultural Chemistry Faculty Of Agriculture Miyazaki University
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Suiko Masahito
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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UMEDA Kazuhiro
Department of Agricultural Chemistry, Miyazaki University
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SUIKO Masahito
Department of Agricultural Chemistry, Miyazaki University
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