Production of S-(Carboxymenthyl)-L-Cysteine from L-Serine with Tryptophan Synthase

概要

論文の詳細を見る
Synthesis of S-(carboxymethyl)-L-cysteine (L-CMC) from L-serine with tryptophan synthase was studied. In the β-replacement reaction with L-serine catalyzed by tryptophan synthase of Escherichia coli, thioglycolic acid was a poor substrate but alkylthioglycolates were good substrates. S-(2-Ethylhexyloxycarbonylmethyl)-L-cys-teine was synthesized from L-serine and 2-ethylhexylthioplycolate using intact cells of recombinant E. coli with high tryptophan synthase activity, and subsequently hydrolyzed to L-CMC with alkali in one reactor. Under optimum conditions established for L-CMC synthesis, 159 g.l^<-1> of L-CMC was formed at a molar yield of 94% based on when both L-serine and 2-ethylhexylthioglycolate were added. Synthesized L-CMC was confirmed to be L-form after isolation and identification of the product.
公益社団法人日本生物工学会の論文
1989-08-25