Cloning and Sequencing of Bacillus stearothermophilus Tryptophan Synthase Genes(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• The tryptophan synthase genes, trpA and trpB, of Bacillus stearothermophilus IFO13737 were cloned by transformation of tryptophan auxotrophic mutations of the trp genes into Escherichia coll. The genes are located in the order of trpB and trpA, according to their coding orientation, in a 2.5 kb EcoRV-HindIII DNA fragment. The complete nucleotide sequence of this DNA was determined. The trpA and trpB genes consist of 810bp (269 amino acid residues) and 1215bp (404 amino acid residues), respectively. The 5'-proximal portion of the trpB gene was found to overlap 20 nucleotides of the upstream coding region of the trpA gene. The homology of the amino acid sequences of the trp gene products of trpA and trpB of B. stearothermophilus is 35 and 50%, respectively, to those of E. coli, and 55 and 70%, respectively, to those of B. subtilis.

• 社団法人日本農芸化学会の論文
• 1989-11-23

著者

• SUZUKI Tadashi

  Central Research Laboratory, Hitachi Ltd.

• Iwamori Satoru

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

• ISHIWATA KEN-ICHI

  Central Research Institute, Mitsui Toatsu Chemicals Inc.,

• MAKIGUCHI NOBUYOSHI

  Central Research Institute, Mitsui Toatsu Chemicals Inc.,

• Ishiwata Ken-ichi

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

• MAKIGUCHI NOBUYOSHI

  Life Science Laboratory, Mitsui Toatsu Chemicals Inc.

• YOSHINO Setsuo

  Central Research Institute, Mitsui Toatsu Chemicals Inc.

• IWAMORI Satoru

  Central Research Institute, Mitsui Toatsu Chemicals Inc.

• Yoshino Shigeru

  Life Science Laboratory Mitsui Toatsu Chemicals Inc.

• Suzuki Tadashi

  Central Research Institute Mitsui Toatsu Chemicals Inc.

関連論文

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• Production of S-(Carboxymenthyl)-L-Cysteine from L-Serine with Tryptophan Synthase
• Enzymatic Production of L-Cysteine with Tryptophan Synthase of Escherichia coli
• Structure of Tyrosine Phenol-Lyase Genes from Citrobacter freundii and Structural Comparison with Tryptophanase from Escherichia coli
• Cloning and Sequencing of Bacillus stearothermophilus Tryptophan Synthase Genes(Microbiology & Fermentation Industry)
• Cross-Flow Filtration of Methane Fermentation Broth by Ceramic Membranes

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