Structure of Tyrosine Phenol-Lyase Genes from Citrobacter freundii and Structural Comparison with Tryptophanase from Escherichia coli
スポンサーリンク
概要
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The tyrosine phenol-lyase (TPL) gene of Citrobacter freundii was cloned and the complete nucleotide sequence of the TPL gene was determined. The TPL gene consists of 1368bp (456 amino acids). The deduced amino acid sequence of TPL was compared with that of tryptophanase from Eschericia coli. A lot of regions are highly conserved, and total homology was 44%. From the observation of the homology data, the PLP binding site of TPL was predicted and confirmed by site-directed mutagenesis.
- 1991-09-25
著者
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Iwamori Satoru
Life Science Laboratory Mitsui Toatsu Chemicals Inc.
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Ishiwata Ken-ichi
Life Science Laboratory Mitsui Toatsu Chemicals Inc.
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YOSHINO SETSUO
Life Science Laboratory, Mitsui Toatsu Chemicals Inc.
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MAKIGUCHI NOBUYOSHI
Life Science Laboratory, Mitsui Toatsu Chemicals Inc.
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Yoshino Shigeru
Life Science Laboratory Mitsui Toatsu Chemicals Inc.
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- Structure of Tyrosine Phenol-Lyase Genes from Citrobacter freundii and Structural Comparison with Tryptophanase from Escherichia coli
- Cloning and Sequencing of Bacillus stearothermophilus Tryptophan Synthase Genes(Microbiology & Fermentation Industry)
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