Conversion of Geotrichum candidum Endocellulase I to Endocellulase II by Limited Proteolysis(Biological Chemistry)
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概要
- 論文の詳細を見る
A limited proteolysis of Avicel-adsorbable. Avicel-disintegrating endocellulase I (molecular weight 130,000) from Geotrichum candidum with subtilisin yielded a protein (molecular weight 80,000) which proved fully active toward soluble substrates such as CM-cellulose, but lost both the abilities to be adsorbed onto insoluble substrates and to disintegrate the cellulose fibres. An immunological experiment showed precipitin lines between endocellulase I and suptilisin-modified endocellulase in the pattern of partial identity. N-Bromosuccinimide-oxidized endocellulase I lost cellulase activity, but retained its adsorbability onto Avicel. It is suggested that endocellulase I had both the affinity site for adsorbing onto insoluble substrates and the ordinary active site.
- 社団法人日本農芸化学会の論文
- 1988-07-23
著者
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Hayashida Shinsaku
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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Hayashida Shinsaku
Department Of Agricultural Chemistry
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MO Kaiguo
Department of Agricultural Chemistry, Faculty of Agriculture, Kyusyu University
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Mo Kaiguo
Department Of Agricultural Chemistry Faculty Of Agriculture Kyusyu University
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