The Integrative Transformation of Pleurotus ostreatus Using Bialaphos Resistance as a Dominant Selectable Marker
スポンサーリンク
概要
- 論文の詳細を見る
A plasmid pLC-bar containing the bialaphos resistance gene derived from Streptomyces hygroscopicus between the Lentinus edodes ras gene promoter and priA gene terminator was constructed. When protoplasts of Pleurotus ostreatus were mixed with the plasmid DNA in the presence of polyethylene glycol and CaCl_2, bialaphos-resistant colonies were obtained. This indicated that transformation was successful. Southern blot analysis of total DNAs from transformants showed that the introduced plasmid DNA was integrated into the host chromosome and partly rearranged. A plasmid, pLC-GUS, containing the Escherichia coli β-glucuronidase (GUS) gene under the control of the L. edodes ras gene promoter and priA gene terminator was constructed and introduced into protoplasts of P. ostreatus with pLC-bar by co-transformation. Two of 5 transformants obtained as bialaphos-resistant colonies showed two to twenty times higher specific activity of GUS than the recipient. Southern blot analysis of total DNAs from transformants indicated the presence of the GUS gene only in the two transfotmants. These results indicated that co-transformation of P. ostreatus was successful, and that the GUS gene was expressed in P. ostreaths. This trainsformation system will enable us to breed commercial strains of P. ostreatus at the molecular level.
- 社団法人日本農芸化学会の論文
- 1996-03-23
著者
-
Yamasaki Toru
東海大学 農学部
-
Yamazaki Tomio
Department Of Hemostasis And Thrombosis Clinical Research Center Nagoya Medical Center
-
Yamazaki T
Department Of Hemostasis And Thrombosis Clinical Research Center Nagoya Medical Center
-
HIRAYAMA Masao
Bioscience Laboratories, Meiji Seika Kaisha, Ltd.
-
YAMAZAKI Takashi
Department of Astrophysics, School of Science, Nagoya University
-
SHISHIDO Kazuo
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Tech
-
KAJIWARA Susumu
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Tech
-
Yamazaki Takashi
Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno
-
Yanai K
Meiji Seika Kaisha Ltd. Saitama Jpn
-
Yanai Koji
Bio Science Laboratories Meiji Seika Kaisha Ltd.
-
Shishido Kazuo
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Hirayama M
Meiji Seika Kaisha Ltd. Saitama Jpn
-
Hirayama Masao
Bio Science Laboratories Meiji Seika Kaisha Ltd.
-
YONEKURA Kumiko
Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
-
USAMI Hideki
Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
-
ADACHI Takashi
Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
-
Usami Hideki
Health & Bioscience Laboratories Meiji Seiko Kaisha Ltd.
-
Yonekura Kumiko
Bio Science Laboratories Meiji Seika Kaisha Ltd.
-
YAMAZAKI Takashi
College of Science and Technology, Nihon University
-
Kajiwara S
Tokyo Inst. Technol. Yokohama Jpn
-
Kajiwara Susumu
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Yamazaki T
Tsukuba Research Institute Ohta's Isan Co. Ltd.
-
Adachi Takashi
Bio Science Laboratories Meiji Seika Kaisha Ltd.
-
Yamazaki Takashi
Department Of Applied Chemistry Graduate School Of Engineering Tokyo University Of Agriculture And T
-
Adachi Takashi
Bio Sci. Lab. , Meiji Seika
関連論文
- Microbial transformation and bioactivation of isoflavones from Pueraria flowers by human intestinal bacterial strains
- Activated protein C resistance in the Japanese population due to homozygosity for the factor V R2 haplotype
- Simultaneous analysis of isoflavones and saponins in Pueraria flowers using HPLC coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside
- A new diterpenoid and a new triterpenoid glucosyl ester from the leaves of Callicarpa japonica Thunb. var. luxurians Rehd.
- A new lignan glucoside from the stems of Callicarpa japonica Thunb. var. luxurians Rehd.
- Five New Diterpenoids, Viteagnusins A-E, from the Fruit of Vitex agnus-castus
- A new phenylethanoid glycoside from the fruits of Callicarpa japonica Thunb. var. luxurians Rehd.
- Gastroprotective effects of bitter principles isolated from Gentian root and Swertia herb on experimentally-induced gastric lesions in rats
- HPLC Profile Analysis of Hepatoprotective Oleanene-Glucuronides in Puerariae Flos
- DPPH radical-scavenging effect on some constituents from the aerial parts of Lippia triphylla
- Two new lignan glucosides from the fruit of Vitex cannabifolia
- Structural Organization and Promoter Activity of the Human Ryudocan Gene
- Molecular Cloning, Genomic Organization, Promoter Activity, and Tissue-Specific Expression of the Mouse Ryudocan Gene^1
- Analysis for heterozygosity of protein S mRNA: application to genetic screening and family studies in hereditary protein S deficiency
- Inulo-Oligosaccharides : Continuous Production from Inulin by Immobilized Inulinase from Aspergillus niger and in vitro Utilization by Bifidobacteria
- Thirty-Meter X-Ray Pencil Beam Line at the Institute of Space and Astronautical Science
- Intron-Dependent Accumulation of mRNA in Coriolus hirsutus of Lignin Peroxidase Gene the Product of Which Is Involved in Conversion/Degradation of Polychlorinated Aromatic Hydrocarbons
- Isolation of a ras gene from the basidiomycete Coriolus hirsutus and use of its promoter for the expression of Pleurotus ostreatus manganese(II) peroxidase cDNA in C. hirsutus
- Studies on the Mechanism of Action of the Gastric H^+,K^+-ATPase Inhibitor SPI-447
- The X-Ray Telescope on board ASCA
- Clinical use of a newly developed calcium phosphate cement (XSB-671D)
- Laser Surgery of Basal, Orbital and Ventricular Meningiomas which are Difficult to Extirpate by Conventional Methods
- Polarization of Protons Scattered from Complex Nuclei
- EPR Studies on the Photo-Induced Intermediates of Ferric NO Complexes of Rat Neuronal Nitric Oxide Synthase Trapped at Low Temperature
- Spinal stenosis in grade I degenerative lumbar spondylolisthesis : a comparative study of outcomes following laminoplasty and laminectomy with instrumented spinal fusion
- Efficacy of tricyclic antidepressant for somatoform pain disorders with chronic lower back and leg pain
- Nucleotide Sequences and Organization of the Genes for Carotovoricin (Ctv) from Erwinia carotovora Indicate That Ctv Evolved from the Same Ancestor as Salmonella typhi Prophage
- Ferroelectric Liquid Crystal Mixtures Containing Chiral Pyranose Compounds for Response-Voltage Minimum Mode
- Transformation of the Edible Basidiomycete Lentinus edodes by Restriction Enzyme-Mediated Integration of Plasmid DNA
- Basidiomycete Fungal Gene Encoding a Regulatory Subunit A Homologue of Protein Phosphatase 2A
- Agrobacterium-mediated transformation of the ectomycorrhizal basidiomycete Tricholoma matsutake that produces commercially valuable fruit bodies, matsutake
- A recQ Gene Homology from the Basidiomycetous Mushroom Lentinula edodes : Sequence Analysis and Expression
- Use of Molecular-genetically Bred Coprinus cinereus Strains for an Efficient Isolation of Cellulose from Rice Straw(Microbiology & Fermentation Technology)
- Structure and Function in Escherichia coli of Plasmids Containing Pyrimidine/ Purine-Biased Stretch Originated from the 5'-Flanking Region of the Basidiomycete ras Gene
- The Integrative Transformation of Pleurotus ostreatus Using Bialaphos Resistance as a Dominant Selectable Marker
- Effect of hyperthermia by magnetite cement on tumor-induced bone destruction
- Efficient Conversion of L-Tryptophan to Indole-3-Acetic Acid and/or Tryptophol by Some Species of Rhizoctonia
- Detection OFIAA BIOSYNTHESIS IN SOME SPECIES OF RHIZOCTONIA
- Characterization of a Water-soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4
- Elastic Scattering of Alpha Particles by ^Ne and ^Mg
- Scattering and Polarization of 57-MeV Protons
- Elastic Scattering of 5.7Mev Protons
- Release of a Soluble form of Growth Hormone Receptors (Growth Hormone-Binding Proteins) from Human IM-9 Cells by Proteolytic Cleavage
- Phosphopeptides in Rat Intestinal Digests after Ingesting Casein Phosphopeptides
- HPLC Analysis of Commercial Casein Phosphopeptides (CPP)
- Retrieval process of high-resolution HAADF-STEM images
- Simulation of atomic-scale high-angle annular dark field scanning transmission electron microscopy images
- Solar Light Absorption Property of Sputtered Al-N Films with Enhanced Surface Roughness during Film Growth(Surfaces, Interfaces, and Films)
- How Do Neutrinos Propagate? : Wave-Packet Treatment of Neutrino Oscillation
- Suppressive Effect of Fructooligosaccharides on the Generation of Volatile Methanethiol in a Fecal Incubation Method and Human Feces
- Brain and Heart Specific Alteration of Methamphetamine (MAP) Distribution in MAP-Sensitized Rat
- Quantitative and easy estimation of a crystal bending effect using low-order CBED patterns
- A new method for dynamical HOLZ calculations
- Role of the α subunit of heterotrimeric G-protein in probenazole-inducing defense signaling in rice
- Possible Role of Phytocassane, Rice Phytoalexin, in Disease Resistance of Rice against the Blast Fungus Magnaporthe grisea(Biochemistry & Molecular Biology)
- Generation of Basidiomycetous Hyphal Cell-aggregates by Addition of the Arg-Gly-Asp Motif-containing Fragment of High-molecular-weight Cell-adhesion Protein MFBA Derived from the Basidiomycete Lentinus edodes
- Effect of Molecular Structure of Chiral Fluorinated Compounds on Spontaneous Polarization of Ferroelectric Liquid Crystals
- Bacillus subtilis Strains Carry Highly Homologous Direct Repeat Sequences on Their Chromosomes
- Sequence Analysis and Expression of a Blue-Light Photoreceptor Gene, Le.phrA from the Basidiomycetous Mushroom Lentinula edodes
- The homologue of Lentinula edodes ctg1, a target for CDC5 and its interacting partner CIPB, from Coprinopsis cinerea is involved in fruiting-body morphogenesis of C. cinerea
- Elastic Scattering of Alpha Particles by ^O
- Elastic and Inelastic Scattering of 22.2 MeV Alpha Particles from Some Medium-Weight Nuclei
- Sequence Analysis and Expression of a Blue-Light Photoreceptor Gene, Le.phrA from the Basidiomycetous Mushroom Lentinula edodes
- Novel Ferroelectric Liquid Crystals Derived from Trifluoromethylated Pyranoses Showing Very Large Spontaneous Polarization
- Synthesis and Properties of Pyranose Derivatives with Trifluoromethyl Group Used as Chiral Dopants for Ferroelectric Liquid Crystals
- Neutrophil-mediated inflammation in respiratory syncytial viral bronchiolitis
- Angular Distribution of Protons Inelastically Scattered from Si^ and Mg^
- Elastic Scattering of 5.7 Mev Protons from Fe, Cr and Ti
- Elicitor Activity of Cerebroside, a Sphingolipid Elicitor, in Cell Suspension Cultures of Rice
- Analysis of mfbA Alleles of the Eubasidiomycete Lentinus edodes
- Effect of Bifidobacterium-Monoassociation and Feeding of Fructooligosaccharides on Lethal Activity of Enterohemorrhagic Escherichia coli O157 in Germ-Free Mice
- Genetic map of a basidiomycete fungus, Lentinula edodes (shiitake mushroom), constructed by tetrad analysis
- Simultaneous analysis of isoflavones and saponins in Pueraria flowers using HPLC coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside
- Mossbauer Spectra of FePS_3 and Its Intercalation Compounds
- Comparison of the Immunopotentiating Activity with Structural Characteristics among Water-soluble Polysaccharides Isolated from the Genus Bifidobacterium
- IAA Biosynthetic Pathway from Tryptophan via Indole-3-pyruvic Acid in Enterobacter cloacae(Microbiology & Fermentation Industry)
- Purification and Properties of a Fructooligosaccharide-producing β-Fructofuranosidase from Aspergillus niger ATCC 20611(Biological Chemistry)
- A Fructooligosaccharide-producing Enzyme from Aspergillus niger ATCC 20611(Biological Chemistry)
- Biological and inherited characteristics of a newly identified Lentinula edodes strain capable of forming a fruiting body without a shift to low temperature
- Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le. CYP1 Enzyme Produced in Yeast
- Cloning and Expression of Cytochrome P450 Genes, Belonging to a New P450 Family, of the Basidiomycete Lentinula edodes(Biochemistry & Molecular Biology)
- Analysis of recQ Gene Transcript in Fruiting Bodies of Basidiomycetous Mushroom Lentinula edodes
- Long Terminal Repeat of the Mushroom Retrotransposon Functions as a Dual Terminator to Transcription
- Distribution of Hydrophobin 1 Gene Transcript in Developing Fruiting Bodies of Lentinula edodes(Biochemistry & Molecular Biology)
- Molecular Cloning and Characterization of the Fructooligosaccharide-Producing β-Fructofuranosidase Gene from Aspergillus niger ATCC 20611
- The (α, p) Reactions on Light and Medium-Weight Nuclei
- Angular Distributions of (α, n) Reaction son Be and C
- Angular Distribution of (α, p) Reactions in Some Light Nuclei
- GENETIC ANALYSIS OF TETRACYCLINE RESISTANCE PLASMID pTP-5
- Preparation and Conformational Study of Fluoroproline-containing Proctolins
- Effect of Dehydrated Nystose Addition on the Reduction of Water Activity
- Determination of the DNA-Binding Sequences of the Zn(II)2Cys6 Zinc-Cluster-Containing PRIB Protein, Derived from the Basidiomycete Lentinus edodes Gene
- Target Genes of the Developmental Regulator PRIB of the Mushroom Lentinula edodes
- A fruiting body-specific novel cDNA, mfbBc, containing continuous 5'-CCA(A/C)CA direct repeats within the coding region, derived from the basidiomycete Lentinula edodes
- Purification and Characterization of Two DNA-Binding Proteins from the Basidiomycete Lentinus edodes(Biological Chemistry)
- Highly Efficient Synthetic Method of Optically Active 1, 1, 1-Trifluoro-2-alkanols by Enzymatic Hydrolysis of the Corresponding 2-Chloroacetates
- Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate
- No Significant Homology of Mitochondrial Genomic DNAs between Basidiomycetes
- Effects of Fructooligosaccharides on the Absorption of Magnesium in the Magnesium-Deficient Rat Model.
- Structure and Function in Escherichia coli of Plasmids Containing Pyrimidine/Purine-Biased Stretch Originated from the 5'-Flanking Region of the Basidiomycete ras Gene.