Purification and Properties of a Fructooligosaccharide-producing β-Fructofuranosidase from Aspergillus niger ATCC 20611(Biological Chemistry)

概要

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A fructooligosaccharide-producing β-fructofuranosidase was purified from cells of Aspergillas niger ATCC 20611. The molecular weight was 340,000 by gel filtration. The optimum pH of the enzyme was 5.0〜6.0 and the optimum temperature was 50〜60℃. The enzyme was rendered inactive by 1 mM Hg^<2+> and the Km value for sucrose was 0.29 M. The enzyme catalyzed an almost exclusively fructosyl transfer reaction in a 50% sucrose solution to produce a mixture of fructooligosaccharides and glucose, but both fructosyl transfer and hydrolytic action were observed in a 0.5% solution. The β-fructofuranosidase showed a high regiospecificity to transfer the fructosyl moiety for the 1-OH group of terminal fructofuranosides.
社団法人日本農芸化学会の論文
1989-03-23