Cloning and Expression of Cytochrome P450 Genes, Belonging to a New P450 Family, of the Basidiomycete Lentinula edodes(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Three cytochrome P450 genes, named Le. cyp1, Le. cyp2, and Le. cyp3, were isolated from the basidiomycete Lentinula edodes. Le. cyp1 and Le. cyp2 contained coding regions of 1500 bp and 1497 bp, respectively, but Le. cyp3 was found to be a defective gene. The deduced amino acid sequences of Le. CYP1 (CYP510A1, 500 amino acids) and Le. CYP2 (CYP510A3, 499 amino acids) were highly similar to each other (87% identical) and they had 32-33% identities to that of Coprinus cinereus P450 (CYP502) and 27-28% identities to those of two Aspergillus P450s (CYP64 family). Quantitative RT-PCR analysis of the transcripts of Le. cyp1 and Le. cyp2 genes in the course of fruiting-body development of L. edodes showed that the primordium seems to contain larger amounts of these transcripts. The transcript levels of both of these genes in the stipe of the premature fruiting body were higher than those in the whole pileus and gill tissue.
- 社団法人日本農芸化学会の論文
- 2002-10-23
著者
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SATO Yoshihiro
Department of Materials Science and Engineering, Faculty of Engineering, Osaka University
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Akiyama Ryoko
Dep. Of Life Sci. Graduate School Of Bioscience And Biotechnology Tokyo Inst. Of Technol.
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Shishido Kazuo
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Kajiwara S
Tokyo Inst. Technol. Yokohama Jpn
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Kajiwara Susumu
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Akiyama Ryoko
Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno
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Sato Y
Dep. Of Life Sci. Graduate School Of Bioscience And Biotechnology Tokyo Inst. Of Technol.
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Sato Yoshihiro
Department Of Materials Science And Engineering Faculty Of Engineering Osaka University
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Sato Yoshihiro
Department Of Biochemistry Kyoritsu College Of Pharmacy
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Sato Yoshihiro
Department of Applied Biological Science, Tokyo University of Science
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