A Stearoyl-CoA-specific Δ9 Fatty Acid Desaturase from the Basidiomycete Lentinula edodes(Biochemistry & Molecular Biology)
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概要
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A gene encoding a Δ9 fatty acid desaturase (Δ9-desaturase) was isolated from L.edodes. The open reading frame of this gene (named Le-FAD1) consisted of 1416 bp. The predicted protein of 471 amino acids shares 47-57% sequence identity with Δ9-desaturases from other fungi. Expression of the Le-FAD1 ORF complemented genetic disruption of the Saccharomyces cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis of the Le-FAD1-expressing S.cerevisiae ole1-disrupted strain showed that Le-FAD1 protein had slight activity with palmitic acid (16 : 0) as the substrate and high activity with stearic acid (18 : 0). Analysis of transcriptional expression showed that the levels of Le-FAD1 mRNA in the primordium and fruiting body of L.edodes were higher than in mycelia cultivated at 18℃ as well as 25℃. These results correlated with the increase of unsaturated fatty acids (UFAs) in total lipids during fruiting-body formation.
- 社団法人日本農芸化学会の論文
- 2003-11-23
著者
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SAKAI Hiromichi
Department of Atomic Science and Nuclear Engineering, Faculty of Engineering, Hokkaido University
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Kajiwara Susumu
Department Of Life Science Graduate School Ofbioscience And Biotechnology Tokyo Institute Of Technol
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Kajiwara Susumu
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Sakai Hiromichi
Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno
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