Yeast Δ12 Fatty Acid Desaturase : Gene Cloning, Expression, and Function

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概要

• 論文の詳細を見る

• In an effort to elucidate the molecular mechanisms of fatty acid desaturation in yeast, a complete gene encoding Δ12 fatty acid desaturase of Saccharomyces kluyveri was cloned. The open reading frame of this gene (named Sk-FAD2) consists of 1,251 bp, encoding 416 amino acids. The deduced Sk-FAD2 protein had 37–55% identity with those from other filamentous fungi. Unlike the genes of these other fungi, S. cerevisiae expressing Sk-FAD2 was found to be capable of synthesizing the dienoic fatty acid hexadecadienoic acid as well as linoleic acid. Moreover, the Sk-FAD2-disrupted strain of S. kluyveri was unable to produce polyunsaturated fatty acids. These results suggested that Sk-FAD2 protein is a unique Δ12 fatty acid desaturase in S. kluyveri. Analysis of transcriptional expression revealed that Sk-FAD2 was not repressed by exogenous unsaturated fatty acids but responded to low-temperature stress.

• 社団法人 日本農芸化学会の論文
• 2004-03-23

著者

• SAKAI Hiromichi

  Department of Atomic Science and Nuclear Engineering, Faculty of Engineering, Hokkaido University

• Kajiwara Susumu

  Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Watanabe K

  Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno

• Watanabe Kyoko

  Department Of Basic Space Science Japan Aerospace Exploration Agency

• Kajiwara Susumu

  Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno

• OURA Takahiro

  Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Tech

• Oura Takahiro

  Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno

• Oura Takahiro

  Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno

• Sakai Hiromichi

  Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno

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