Substrate Specificity and Regioselectivity of Δ12 and ω3 Fatty Acid Desaturases from Saccharomyces kluyveri
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概要
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Δ12 and ω3 fatty acid desaturases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs), which are important constituents of membrane glycerolipids and also precursors to signaling molecules in many organisms. In this study, we determined the substrate specificity and regioselectivity of the Δ12 and ω3 fatty acid desaturases from Saccharomyces kluyveri (Sk-FAD2 and Sk-FAD3). Based on heterologous expression in Saccharomyces cerevisiae, it was found that Sk-FAD2 converted C16–20 monounsaturated fatty acids to diunsaturated fatty acids by the introduction of a second double bond at the ν+3 position, while Sk-FAD3 recognized the ω3 position of C18 and C20. Furthermore, fatty acid analysis of major phospholipids suggested that Sk-FAD2 and Sk-FAD3 have no strong substrate specificity toward the lipid polar head group or the sn-positions of fatty acyl groups in phospholipids.
- 社団法人 日本農芸化学会の論文
- 2008-12-23
著者
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Kajiwara Susumu
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Kajiwara Susumu
Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno
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OURA Takahiro
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Tech
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Oura Takahiro
Department Of Life Science Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Techno
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