Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting Green Emission before and Red Emission after Cre-mediated Recombination

スポンサーリンク

概要

• 論文の詳細を見る

• The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression in vivo. In most Cre-reporter mouse strains, although the presence of reporter product indicates the expression of Cre recombinase, it has remained unclear whether a lack of reporter signal indicates either no Cre recombinase expression or insufficient reporter gene promoter activity. We produced a novel ROSA26 knock-in Cre-reporter C57BL/6N strain exhibiting green emission before and red after Cre-mediated recombination, designated as strain R26GRR. Ubiquitous green fluorescence and no red fluorescence were observed in R26GRR mice. To investigate the activation of tdsRed, EGFP-excised R26GRR, R26RR, mice were produced through the crossing of C57BL/6N mice with R26GRR/Ayu1-Cre F1 mice. R26RR mice showed extraordinarily strong red fluorescence in almost all tissues examined, suggesting ubiquitous activation of the second reporter in all tissues after Cre/loxP recombination. Moreover, endothelial cell lineage and pancreatic islet-specific expression of red fluorescence were detected in R26GRR/Tie2-Cre F1 mice and R26GRR /Ins1-Cre F1 mice, respectively. These results indicated that R26GRR mice are a useful novel Cre-reporter mouse strain. In addition, R26GRR mice with a pure C57BL/6N background represent a valuable source of green-to-red photoconvertible cells following Cre/loxP recombination for application in transplantation studies. The R26GRR mouse strain will be available from RIKEN BioResource Center (http://www.brc.riken.jp/lab/animal/en/).

• Japanese Association for Laboratory Animal Scienceの論文

著者

• YAGAMI Ken-ichi

  Laboratory Animal Research Center

• YOSHIKI Atsushi

  RIKEN BioResource Center

• TAKAHASHI Satoru

  Laboratory Animal Resource Center

• KAJIWARA Noriko

  Laboratory Animal Research Center, University of Tsukuba

• MEKADA Kazuyuki

  RIKEN BioResource Center

• Mizuno Seiya

  Laboratory Animal Resource Center University Of Tsukuba

• Sugiyama Fumihiro

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Hasegawa Yoshikazu

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Daitoku Yoko

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Sekiguchi Keito

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Tanimoto Yoko

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Mizuno-Iijima Saori

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Ema Masatsugu

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Miwa Yoshihiro

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Kajiwara Noriko

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Yagami Ken-ichi

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Yoshiki Atsushi

  RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan

関連論文

• Vascular Remodeling in Hypertensive Transgenic Mice
• 組換え抗原を用いたマウスパルボウイルス特異的なELISA法の開発
• C57BL/6亜系統間の遺伝的相違について
• 理研バイオリソースセンターのマウスリソース
• Chromosomal Mapping of Human Angiotensinogen Gene and Human Renin Gene by Fluorescence In Situ Hybridization (FISH) in Transgenic Mice
• Elimination of Pasteurella pneumotropica from a Contaminated Mouse Colony by Oral Administration of Enrofloxacin
• Prevalence of "Orphan" Parvovirus Infections in Mice and Rats
• IS-10 Considerations on the Cause of Anorectal Malformations : Involvement of cell proliferation and differentiation
• Vertical Transmission to Embryo and Fetus in Maternal Infection with Rat Virus (RV)
• A-type Natriuretic Peptide Level in Hypertensive Transgenic Mice
• 日本産野生マウスに由来する独自の近交系統MSM/Ms
• Retrotransposon-Mediated Fgf5go-Utr Mutant Mice with Long Pelage Hair
• Further evidence for recombination between mouse hemoglobin beta b1 and b2 genes based on the nucleotide sequences of intron, UTR, and intergenic spacer regions
• c-Maf and MafB transcription factors are differentially expressed in Huxley's and Henle's layers of the inner root sheath of the hair follicle and regulate cuticle formation
• I-6 THE SAME EMBRYOGENETIC PROCESS BETWEEN ANTERIOR SACRAL MENINGOCELE AND IMPERFORATE ANUS? : A MOUSE MODEL STUDY
• Development of Genetically Engineered Mice with Hypertension and Hypotension
• Retrotransposon-Mediated Fgf5^ Mutant Mice with Long Pelage Hair
• Pregnancy-associated homeostasis and dysregulation : lessons from genetically modified animal models
• Contribution of the RIKEN BRC to the International Mouse Strain Resource
• Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs
• Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting Green Emission before and Red Emission after Cre-mediated Recombination

もっと見る 閉じる

スポンサーリンク