Basic Subsite Theory Assumptions May Not Be Applicable to Hydrolysis of Cellooligosaccharides by Almond β-Glucosidase
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概要
- 論文の詳細を見る
Steady-state kinetic parameters for the hydrolysis of cellooligosaccharides by almond β-glucosidase were evaluated at pH 5.0 and 25℃ in relation to the subsite theory (K.Hiromi, Biochem.Biophys.Res.Commun., 40, 1-6, 1970). The value of k_0/Κ_m decreased monotonously with increasing degree of polymerization (DP) of the substrates (DP=2-6). Also, the Κ_m and k_0 values for cellotriose were smaller than those for cellobiose. These DP dependencies differ from those of most amylases and glucosidases studied so far, to which the subsite theory has been successfully applied. The subsite parameters could not be consistently obtained, which suggests that one or both of the two basic assumptions of the subsite theory might not be applicable to the hydrolysis of cellooligosaccharides by the enzyme. That is, the intrinsic rate of the hydrolysis may depend on the DP and/or there may be interaction between subsites for binding the glucose residues of a substrate.
- 社団法人日本生物工学会の論文
- 1999-12-25
著者
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Obata H
Graduate School Of Bioresources Mie University
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Obata Hitoshi
Faculty of Bioresources, Mie University
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Tanaka Akiyoshi
Faculty of Bioresources, Mie University
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SENOO Keishi
Faculty of Bioresources, Mie University
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Senoo Keishi
Department Of Applied Biological Chemistry Division Of Agriculture And Life Sciences The University
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Senoo Keishi
Faculty Of Agriculture The University Of Tokyo
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Tanaka A
Faculty Of Bioresources Mie University
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NAKAGAWA CHIKA
Faculty of Education, Mie University
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KODAIRA KAZUKO
Faculty of Education, Mie University
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Kodaira Kazuko
Faculty Of Education Mie University
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Nakagawa Chika
Faculty Of Education Mie University
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Tanaka A
Graduate School Of Bioresources Mie Univ
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Tanaka Akiyoshi
Faculty Of Bioresources Mie University
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Senoo Keishi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Obata Hitoshi
Faculty Of Agriculture Mie University
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