Release of Cell-Free Ice Nuclei from Pseudomonas viridiflava with a Triton X-100/EDTA System and Their Ice Nucleation Properties
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概要
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The release of cell-free ice nuclei from Pseudomonas viridiflava KUIN-2 cells with Triton X-100 in the presence of EDTA-2Na, NaSCN, Na_2SO_4,or KCl was attempted. Cell-free ice nuclei were shed from P. viridiflava KUIN-2 cells with 2% Triton X-100 in the presence of 10 mM EDTA-2Na, KUIN-2 shed cell-free ice nuclei (0.22 mg protein/ml) active at -4.9 to -5.8℃. The ice-nucleating activity was greatly decreased by treating the cell-free ice nuclei for 30 min at 40℃, and was completely lost by heating at 90℃. The ice-nucleating activity of the cell-free ice nuclei was inhibited by the addition of proteases, protein-modifying reagents and denaturants. However, phospholipase C had no such effect. It is suggested that the ice-nucleating activity sites of cell-free ice nuclei contain proteins. The ice-nucleating activity of cell-free ice nuclei containing Mg^<2+> occurred within the temperature range -3.3 to -3.6℃.
- 社団法人日本生物工学会の論文
- 1990-11-25
著者
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Obata Hitoshi
Faculty of Bioresources, Mie University
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Tokuyama Tai
Faculty Of Engineering Kansai University
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TAKEUCHI SEICHI
Faculty of Engineering, Kansai University
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Obata Hitoshi
Faculty Of Agriculture Mie University
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Takeuchi S
Science Univ. Tokyo Chiba Jpn
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