Effects of Zinc Deficiency on Peptide Synthesis in Cultured Tobacco Plant Cells
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概要
- 論文の詳細を見る
To analyze the effects of Zn deficiency on protein synthesis at the translation and transcription levels, proteins and peptides extracted from tobacco cells cultured with (+Zn) and without Zn (-Zn) were compared by two-dimensional electrophoresis. mRNAs were compared by in vitro translation. Although the spots of translated peptides were almost identical for the -Zn and +Zn treatments, one peptide spot at pI 6.0 appeared densely by the -Zn treatment. Clear differences were observed in the separation patterns of water soluble cellular proteins and peptides. In the -Zn treatment, two peptides at pI 4.5 (MW 45,000) and at pI 5.5 (MW 50,000) which were present in the +Zn treatment disappeared while one appeared at pI 6.0 (MW 28,000). Zinc may participate in the formation of some peptides and under Zn deficiency, the transcription of one mRNA for a peptide which appeared at pI 6.0 was enhanced in this study.
- 一般社団法人日本土壌肥料学会の論文
著者
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Umebayashi Masanao
Faculty of Bioresources, Mie University
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Umebayashi M
Faculty Of Bioresources Mie University
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Obata Hitoshi
Faculty Of Agriculture Mie University
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Toda T
Faculty Of Bioresources Mie University:(present Addoress)graduate School Of Science And Engineering
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Umebayashi Masanao
Faculty Of Agriculture Mie University
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Toda Tsutomu
Faculty of Bioresources, Mie University
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