Differential Scanning Calorimetry of the Effects of Ca^<2+> on the Thermal Unfolding of Pseudomonas cepacia Lipase(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• Thermal unfolding of P. cepacia lipase was observed by adiabatic differential scanning microcalorimetry in the absence and presence of calcium ions at pH 8, and thermodynamic parameters of unfolding were evaluated to analyze the unfolding mechanism of the enzyme. The temperature of unfolding was higher at higher concentrations of Ca^<2+>. From the Ca^<2+> concentration-dependence of the unfolding temperature, the number of calcium ions that dissociated from the enzyme molecule upon unfolding was estimated to be one. These results confirmed the validity of the unfolding mechanism proposed previously : Nca^<2+>⇌D + Ca^<2+>, where N and D represent the native and denatured states, respectively, of the enzyme.

• 社団法人日本農芸化学会の論文
• 2003-01-23

著者

• Mizuno Takafumi

  Graduate School Of Bioresources Mie University

• OBATA Hitoshi

  Graduate School of Bioresources, Mie University

• SENOO Keishi

  Graduate School of Agricultural and Life Sciences, University of Tokyo

• Obata H

  Graduate School Of Bioresources Mie University

• Mizuno Takafumi

  Faculty of Bioresources, Mie University

• Obata Hitoshi

  Faculty of Bioresources, Mie University

• Tanaka Akiyoshi

  Faculty of Bioresources, Mie University

• SUGIMOTO Hayuki

  Faculty of Bioresources, Mie University

• MUTA Yuko

  Graduate School of Agriculture, Kyoto University

• INOUYE Kuniyo

  Graduate School of Agriculture, Kyoto University

• Inouye K

  Kyoto Univ. Kyoto Jpn

• Inoue K

  Kansai Medical Univ. Osaka

• Senoo Keishi

  Department Of Applied Biological Chemistry Division Of Agriculture And Life Sciences The University

• Senoo Keishi

  Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Tanaka A

  Faculty Of Bioresources Mie University

• Inouye Kuniyo

  Graduate School Of Agriculture Kyoto University

• Muta Y

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• Inouye K

  Kyoto Univ. Kyoto

• Obata Hitoshi

  Graduate School Of Bioresources Mie Univ.

• Tanaka A

  Graduate School Of Bioresources Mie Univ

• Tanaka Akiyoshi

  Faculty Of Bioresources Mie University

• Senoo Keishi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Obata Hitoshi

  Faculty Of Agriculture Mie University

• Sugimoto Hayuki

  Faculty Of Bioresources Mie University

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