Identification of a Novel Anti-ice-nucleating Polysaccharide from Bacillus thuringiensis YY529(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Strain YY529, capable of producing some anti-ice-nucleating materials (ANM), was isolated from the surface of a camphor leaf. Strain YY529 was identified as Bacillus thuringiensis from its characteristics and taxonomy ; the optimum temperature and pH for producing these ANMs were 30℃ and 7.0, respectively. One of the ANM with the highest activities among them was purified from the culture. The molecular weight of the ANM was approximately 130 kDa based on a gel filtration analysis. We confirmed that this ANM was a polysaccharide based on the results of the treatment with a mannosidase and the molish reaction. In addition, the LCMS analysis showed that this anti-ice-nucleating polysaccharide (ANPS) had the polyacetyl-D-glucosamine moiety in its structure. Furthermore, this ANPS showed its ability as a non-freeze agent in a preservative solution for the cryopreservation of cock liver. This is the first report of ANPS as a novel ANM from Bacillus thuringiensis YY529.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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OBATA Hitoshi
Graduate School of Bioresources, Mie University
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Obata H
Graduate School Of Bioresources Mie University
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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YAMASHITA YASUHIRO
Department of Pediatrics and Child Health, Kurume University School of Medicine
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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Yamashita Yasushi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamashita Yasuhiro
Department Of Chemistry School Of Science The University Of Tokyo
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Yamashita Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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Yamashita Yasuhiro
Department of Biotechnology, Faculty of Engineering, Kansai University
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