Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium, Pseudomonas fluorescens KUIN-1
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概要
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The psychrotrophic ice-nucleating bacterium, Pseudomonas fluorescens KUIN-1 respond to a decrease in temperature with the inductioin of proteins that are classified as cold shock proteins (CSPs). We found the function of a 26-kDa protein of the CSPs in the strain KUIN-1. In strain KUIN-1,a cold shock from 18 to 4℃ induced the synthesis of the 26-kDa protein. By analysis with SDS-PAGE, it was then demonstrated that the 26-kDa protein was produced by the cells after treatment at 4℃. The 26-kDa protein was purified to apparent homogeneity by (NH_4)_2SO_4 precipitation and some chromatographies (QA52,phenyl Superose, Superose 12,and Mono Q). The purified 26-kDa protein is composed of 6 subunits of 26.5-kDa with a molecular mass of approximately 159-kDa according to gel filtration and SDS-PAGE. The N-terminal sequence of the 26-kDa protein was Gln-Ala-Ala-Tyr-Tyr-Pro-Ala-His-His-His-Gln-Gln-Val-Gln-Gln-His-Trp-Gly-His-His-. Specifically, 26-kDa protein of the CSPs of strain KUIN-1 was very effective in protecting the cold-labile enzyme, lactate dehydrogenase against denaturation by freezing. The characteristics of 26-kDa protein are analogous to the cold-regulated protein of the plants.
- 社団法人日本農芸化学会の論文
- 1998-11-23
著者
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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YAMADE KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
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ISHIGAKI Hitoshi
Department of Biotechnology, Faculty of Engineering, Kansai University
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Yamade K
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamade Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University:kansai University High Technolo
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Ishigaki Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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