Purification and Characterization of Extracellular Ice-nucleating Matter from Erwinia uredovora KUIN-3
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概要
- 論文の詳細を見る
The extracellular ice-nucleating matter (EIM) produced by the ice-nucleating bacterium, Erwinia uredovora KUIN-3, was purified to apparent homogeneity by ultrafiltration, sucrose density-gradient ultracentrifugation, and gel filtration. The purified EIM was sphercial matter (0.2-0.4 μm) by examination using a transmission electron microscope. The ice-nucleating activity from the EIM was equal to that of the cells in this strain. It had become apparent that the EIM had class A (〜 -5℃), B (-5 〜 -8℃), and C (-8℃ 〜) structures as judged by its freezing difference spectrum in D_2O versus H_2O. The enzyme treatments by protease K, α-mannosidase, β-galactosidase, and phospholipase C decreased the activity of the EIM as well as of the cells. Further, the activities of class A and B from the EIM were decreased by treatment with diamine oxidase. Also, the activity of the purified. EIM was stable from pH 5.0 to 10.0 and at temperatures below 25℃. It was demonstrated that the components of the EIM were lipid (10%), protein (43%), saccharide (35%), and polyamine (12%).
- 社団法人日本農芸化学会の論文
- 1993-09-23
著者
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Mano Yoshinori
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Faculty of Bioresources, Mie University
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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Kawahara Hidehisa
Faculty of Engineering, Kansai University
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Mano Yoshinori
Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Faculty Of Engineering Kansai University
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Obata Hitoshi
Faculty Of Agriculture Mie University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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