Identification of an Antifreeze Lipoprotein from Moraxella sp. of Antarctic Origin(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
We found six bacteria capable of producing antifreeze protein (AFP) from Ross Island, Antarctica. Among these AFP-producing bacteria, strain No. 82 had the highest antifreeze activity and was identified as Moraxella sp. The optimum temperature and pH for the production of AFP were 5℃ and 7.0, respectively. After partially purifying the AFP from the culture supernatant using 60% saturation of ammonium sulfate, only the 52-kDa protein band (100 μg /ml) which eluted from SDS-PAGE indicated antifreeze activity by the formation of hexagonal crystals. Furthermore, we confirmed that this AFP was a lipoprotein by the lipid stain test and treatment with some enzymes and that it had no icenucleating activity. Also, the N-terminal amino acid sequence of this AFP had high similarity with that of outer membrane proteins from Moraxella (Branhamella) catarrhalis. This is the first report of AFP-producing bacteria in Antarctica and an antifreeze lipoprotein (AFLP) from Moraxella sp.
- 社団法人日本農芸化学会の論文
- 2002-02-23
著者
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OBATA Hitoshi
Graduate School of Bioresources, Mie University
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Obata H
Graduate School Of Bioresources Mie University
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Obata H
Department Of Biotechnology Faculty Of Engineering Kansai University
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Obata Hitoshi
Department Of Biotechnology Faculty Of Engineering Kansai University
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YAMASHITA YASUHIRO
Department of Pediatrics and Child Health, Kurume University School of Medicine
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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NISHIKAWA Jiro
Department of Applied Biological Science, Faculty of Science and Technology, Science University of T
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OMIYA KAZUHIRO
Department of Biotechnology, Faculty of Engineering, Kansai University
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NAKAMURA Nofirumi
Department of Biotechnology, Faculty of Engineering, Kansai University
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawahara Hidehisa
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Obata Hitoshi
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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Nishikawa Jiro
Departmant Of Civil And Environmental Engineering Yamanashi University
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Omiya Kazuhiro
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamashita Yasushi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Yamashita Yasuhiro
Department Of Chemistry School Of Science The University Of Tokyo
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Nakamura Norifumi
Department Of Oral And Maxillofacial Surgery Field Of Maxillofacial Rehabilitation Kagoshima Univers
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Yamashita Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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Nakamura Nofirumi
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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Yamashita Yasuhiro
Department of Biotechnology, Faculty of Engineering, Kansai University
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Nakamura Norifumi
Department of Biotechnology, Faculty of Engineering, Kansai University
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