Effects of Zinc Deficiency on the Growth, Proteins, and Other Constituents of Yeast, Saccharomyces cerevisiae, Cells
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概要
- 論文の詳細を見る
To carry out a series of experiments in order to analyze the effects of Zn deficiency on protein synthesis in higher plants, we used a budding yeast, Saccharomyces cerevisiae, as model organism. Yeast cells were cultured in a chemically defined Burkholder minimum medium with and without Zn. When the Zn concentration in the cells decreased to a level of less than 100 mg kg^<-1> dry weight, cell growth was depressed. The cells in the -Zn culture swelled, and after further culture, they formed clusters. Zinc deficiency enhanced the Ca concentration in the cells and did not affect the K and Mg concentrations. Zinc deficiency remarkably depressed the protein content of the cells. Some soluble proteins and peptides disappeared and somo proteins and peptides appeared in the -Zn culture. pI of one protein shifted from 8.0 to 7.8 in the -Zn culture under nondenaturing conditions, and the N-terminal amino acid sequence of this protein showed a 100% homology with enolase (EC 4. 2. 1. 11).
- 一般社団法人日本土壌肥料学会の論文
著者
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Obata Hitoshi
Graduate School Of Bioresources Mie Univ.
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Umebayashi Masanao
Faculty of Bioresources, Mie University
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Umebayashi M
Faculty Of Bioresources Mie University
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Obata Hitoshi
Faculty Of Agriculture Mie University
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Toda T
Faculty Of Bioresources Mie University:(present Addoress)graduate School Of Science And Engineering
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Umebayashi Masanao
Faculty Of Agriculture Mie University
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Hayashi Atsushi
Faculty Of Bioresources Mie University:(present Addoress)nichi Nichi Pharmaceutical Co.ltd
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Toda Tsutomu
Faculty of Bioresources, Mie University
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