Cloning of Bacterial Ice Nucleation Genes of Pseudomonas viridiflava in Escherichia coli
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概要
- 論文の詳細を見る
A cosmid library containing Pseudomonas viridiflava KUIN-2-DNA was constructed in the vector Homer III and used to transform E. coli HB101. Five colonies (INAC-1〜-5) with ice-nucleating activity (INAI) were obtained and all these transformants had in common an approximately 13.2-kb EcoR1-fragment (pNVR-1). Among them, the transformant INAC-2,which harbored a single plasmid (pCH-1) and had an excellent INA, was selected for studying the characteristics of the INA^+ transformant. The highest INA was obtained when INAC-2 was cultured aerobicaliy in Luria broth (pH 7.2) for 15-20 h at 20℃. The INA in culture was not detectable until the bacterial titer reached 10^5 to 10^6 cells/ml. The heat stability of the ice-nucleating substance in the transformant was more thermostable than that in the donor strain. Further, E. coli HB101 harboring the self-circularized pNVR-1 had INA.
- 公益社団法人日本生物工学会の論文
- 1990-09-25
著者
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HASEGAWA YOSHIE
Department of Biotechnology, Faculty of Engineering, Kansai University
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Tokuyama Tai
Faculty Of Engineering Kansai University
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Hasegawa Y
Department Of Biotechnology Faculty Of Engineering Kansai University
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Kawate Shohei
Faculty Of Engineering Kansai University
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Obata Hitoshi
Faculty Of Agriculture Mie University
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HASEGAWA YOSHIE
Faculty of Engineering, Kansai University
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SAKAI NORIHITO
Faculty of Engineering, Kansai University
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YOSHITOME HIRONORI
Faculty of Engineering, Kansai University
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Sakai Norihito
Faculty Of Engineering Kansai University
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Yoshitome Hironori
Faculty Of Engineering Kansai University
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